Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Centre of Excellence in Swine Reproduction, Chulalongkorn University, Bangkok, 10330, Thailand.
Intervet (Thailand) Ltd., South Sathorn Rd., Yannawa, Sathorn, Bangkok, 10120, Thailand.
Theriogenology. 2022 Oct 15;192:45-51. doi: 10.1016/j.theriogenology.2022.08.026. Epub 2022 Aug 24.
The present study was performed to evaluate the reproductive performance of gilts inseminated using fixed-time artificial insemination (FTAI) protocol. A total of 408 Landrace × Yorkshire crossbred gilts were included in the experiment. Gilts at 8 months of age were randomly allocated into three groups: control AI (n = 192), treatment 1-TAI (n = 117) and treatment 2-FTAI (n = 99). Gilts in the control AI group were inseminated 2-3 times during standing oestrus at 0, 12 and 24 h after the onset of oestrus. Gilts in the treatment 1-TAI group were orally administered 20 mg per day of altrenogest for 18 days and then inseminated 2-3 times during standing oestrus by conventional AI. Gilts in the treatment 2-FTAI group were synchronized like gilts in treatment 1-TAI group but then GnRH (10 μg of buserelin) was administered 120 h after the end of altrenogest treatment and fixed time artificial inseminated twice at 24 and 32 h after GnRH irrespective of the presence of oestrus or not. Conception rate of gilts in treatment 2-FTAI (87.9%) was similar to the treatment 1-TAI (94.9%) and control AI (83.3%) (P > 0.05). Conception rate in treatment 1-TAI (94.9%) was higher compared to control AI group (83.3%, P = 0.040). Farrowing rate of gilts in treatment 2-FTAI (83.8%) was similar to treatment 1-TAI (89.7%) and control AI (76.0%) (P > 0.05). Farrowing rate of treatment 1-TAI (89.7%) was higher than control AI gilts (76.0%, P = 0.033). In treatment 2-FTAI, the conception and farrowing rate of the nine gilts that were inseminated even if they were not detected in oestrus (all during warm season) was 44.4% and 44.4%, respectively. Regular return to oestrus was similar between groups (9.4%, 0.9% and 4.1% for control AI, treatment 1-TAI and treatment 2-FTAI, respectively, P > 0.05). The total number of piglets born per litter in treatment 1-TAI group was higher than control AI (13.1 ± 0.2 versus 11.6 ± 0.2, respectively, P < 0.001) and treatment 2-FTAI groups (12.2 ± 0.3, P = 0.019). The number of piglets born alive was higher in treatment 1-TAI (12.1 ± 0.3) compared to treatment 2-FTAI (11.3 ± 0.2) and control AI group (11.2 ± 0.3). The percentage of stillbirth and mummified foetus were not different between groups (P > 0.05). The present study indicated that fixed-time AI in gilts can be successfully performed by administration of altrenogest for 18 days, GnRH at 120 h after altrenogest withdrawal and then double fixed-time AI at 24 and 32 h after the administration of GnRH. Fertility metrics such as conception rate, farrowing rate and litter performances using this method were similar to gilts inseminated at oestrus with conventional AI.
本研究旨在评估采用定时人工授精（FTAI）方案对母猪进行配种的繁殖性能。共有 408 头长白猪×约克夏杂交母猪参与了实验。8 月龄的母猪随机分为三组：对照组 AI（n=192）、处理 1-TAI（n=117）和处理 2-FTAI（n=99）。对照组 AI 母猪在发情开始后 0、12 和 24 小时进行三次定时授精。处理 1-TAI 组母猪每天口服 20 毫克阿尔特龙，并在发情站立期进行三次定时授精。处理 2-FTAI 组母猪与处理 1-TAI 组母猪同步处理，但在阿尔特龙治疗结束后 120 小时给予 GnRH（10μg 布舍瑞林），并在 GnRH 给药后 24 和 32 小时进行两次定时人工授精，无论是否发情。处理 2-FTAI 组母猪的受胎率（87.9%）与处理 1-TAI 组（94.9%）和对照组 AI（83.3%）相似（P>0.05）。处理 1-TAI 组的受胎率（94.9%）高于对照组 AI 组（83.3%，P=0.040）。处理 2-FTAI 组母猪的产仔率（83.8%）与处理 1-TAI 组（89.7%）和对照组 AI（76.0%）相似（P>0.05）。处理 1-TAI 组的产仔率（89.7%）高于对照组 AI 组（76.0%，P=0.033）。在处理 2-FTAI 中，在发情期未检测到的 9 头母猪（均在暖季）的受胎率和产仔率分别为 44.4%和 44.4%。各组的发情周期恢复正常（对照组 AI、处理 1-TAI 和处理 2-FTAI 分别为 9.4%、0.9%和 4.1%，P>0.05）。处理 1-TAI 组的每窝产仔数高于对照组 AI（13.1±0.2 对 11.6±0.2，P<0.001）和处理 2-FTAI 组（12.2±0.3，P=0.019）。处理 1-TAI 组的活产仔数（12.1±0.3）高于处理 2-FTAI 组（11.3±0.2）和对照组 AI 组（11.2±0.3）。各组死产和木乃伊胎的比例无差异（P>0.05）。本研究表明，通过 18 天的阿尔特龙治疗、阿尔特龙停药后 120 小时给予 GnRH 以及随后在 GnRH 给药后 24 和 32 小时进行两次定时人工授精，可以成功对母猪进行定时 AI。使用这种方法的受胎率、产仔率和窝产性能等繁殖指标与发情期常规 AI 授精的母猪相似。
