[一种来自卷柏的新型环肽]
[A new cyclopeptide from Selaginella tamariscina].
作者信息
Yan Xin-Jia, Wen Jing, Song Yang, Sha Dong-Mei, Sha Ma-Li-Niu, Zhang Shao-Shan, Liu Yuan
机构信息
Institute of Qinghai-Tibetan Plateau, Southwest Minzu University Chengdu 610041, China Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China Chengdu 610225, China Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory Chengdu 610225, China.
College of Pharmacy, Harbin University of Commerce Harbin 150076, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2022 Aug;47(16):4391-4394. doi: 10.19540/j.cnki.cjcmm.20220421.204.
One new cyclopeptide was isolated from the ethyl acetate fraction of the 75% EtOH extract of Selaginella tamariscina by various column chromatography methods(HP-20, polyamide and semi-preparative HPLC). Its structure was identified as selapeptin A(1) by extensive spectroscopic analysis(HR-ESI-MS, 1 D and 2 D NMR). Compound 1 was evaluated for cytotoxic activities by MTT assay. It showed potent cytotoxic activity against B16 F10 with the inhibition rate of 51.57%±4.34% at 40 μmol·L(-1) while had no impacts on MDA-MB-231 and MDA-MB-468 at 100 μmol·L(-1).
通过多种柱色谱方法（HP-20、聚酰胺和半制备型高效液相色谱）从卷柏75%乙醇提取物的乙酸乙酯馏分中分离得到一种新的环肽。通过广泛的光谱分析（高分辨电喷雾电离质谱、一维和二维核磁共振）确定其结构为卷柏肽A（1）。采用MTT法对化合物1的细胞毒性活性进行了评价。在40 μmol·L⁻¹时，它对B16 F10显示出较强的细胞毒性活性，抑制率为51.57%±4.34%，而在100 μmol·L⁻¹时对MDA-MB-231和MDA-MB-468没有影响。
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