Department of Biomedical Engineering, Graduate School of Science and Technology, Shinshu University, Wakasato 4-17-1, Nagano-shi, Nagano 380-8553, Japan.
Institute for Advanced Biosciences, Keio University, Nipponkoku, Tsuruoka, Yamagata 997-0017, Japan.
J Biochem. 2022 Oct 19;172(5):313-319. doi: 10.1093/jb/mvac069.
In this study, a Bacillus natto strain named NEST141 was constructed. The strain carries no plasmids and is an authentic proline auxotroph-a feature that confers effective selection conditions for plasmids transferred from a donor, such as Bacillus subtilis 168, via a pLS20-based conjugational transfer system. We have provided a standard effective protocol for the delivery of plasmids larger than 50 kilobase pairs. These results indicate that the B. natto NEST141 strain can become a standard model, like B. subtilis 168, for extensive genetic engineering with diverse applications.
在这项研究中，构建了一株纳豆芽孢杆菌菌株，命名为 NEST141。该菌株不携带质粒，是真正的脯氨酸营养缺陷型，这一特性为通过基于 pLS20 的共轭转移系统从供体（如枯草芽孢杆菌 168）转移的质粒提供了有效的选择条件。我们提供了一个标准的有效方案，用于传递大于 50 千碱基对的质粒。这些结果表明，纳豆芽孢杆菌 NEST141 菌株可以成为一个标准模型，类似于枯草芽孢杆菌 168，用于广泛的遗传工程，具有多种应用。
