猫新鲜冷冻血浆在储存 1 年后用于输血时凝血因子的稳定性。
Stability of coagulation factors in feline fresh frozen plasma intended for transfusion after 1 year of storage.
机构信息
Department of Animal Medicine and Surgery, Veterinary Faculty, Autonomous University of Barcelona, Barcelona, Spain.
Animal Blood Bank, Barcelona, Spain.
出版信息
J Feline Med Surg. 2022 Oct;24(10):e353-e359. doi: 10.1177/1098612X221114630. Epub 2022 Sep 1.
OBJECTIVES
The most common use of plasma transfusion is for haemostatic purposes, but coagulation factor activities in stored feline plasma are unknown. The concentration and stability of coagulation factors I (fibrinogen), II, V, VII, VIII, IX, X, XI and XII in feline fresh frozen plasma (fFFP) stored for 1 year were studied.
METHODS
Fifty-five units of fFFP were produced from 55 fresh whole-blood donations obtained from indoor healthy blood donor cats. Twenty-one units were stored for <2 weeks (T0) and 34 were stored for 1 year (T1). After the completion of storage, specific coagulation factor activities for factors II, V, VII, VIII, IX, X, XI and XII were tested using modified one-stage activated partial thromboplastin or prothrombin time assays. Fibrinogen was determined using the Clauss method.
RESULTS
Significantly decreased activities were observed for factors II (T0: 101.94% ± 19.06%; T1: 73.23% ± 39.06% [ = 0.001]), VII (T0: 102.78% ± 24.69%; T1: 60.08% ± 38.17% []), VIII (T0: 77.52% ± 30.39%; T1: 50.32% ± 23.8% [ = 0.001]), XI (T0: 88.76% ± 22.73%; T1: 66.28% ± 22.2% [ = 0.001]) and XII (T0: 89.50% ± 21.85%; T1: 55.46% ± 23.18% [ <0.001]) when comparing units at time 0 and after 1 year of storage. No significant difference was observed for factors IX (T0: 84.86% ± 29.35%; T1: 71.37% ± 22.23% [ = 0.064]) and X (T0: 96.24% ± 25.1%; T1: 83.91% ± 49.54% [ = 0.236]). Unexpectedly, a significant increase was observed for factor V (T0: 71.94% ± 24.14%; T1: 97.89% ± 62.33%; = 0.046). Fibrinogen was 2.76 ± 1.09 g/l at T1. Factors VIII, XII and VII had the lowest mean activities after 1 year.
CONCLUSIONS AND RELEVANCE
Although a decrease in most coagulation factors activities was noted with storage, 1-year-old fFFP was haemostatically active in vitro. The most suitable factors for quality control assessment of fFFP are factors VII and VIII. Approximately 13-20 ml/kg of fFFP is required to administer a minimum of 10 IU/kg coagulation factor activity.
目的
血浆输注最常用于止血目的，但储存的猫血浆中的凝血因子活性尚不清楚。本研究旨在研究储存 1 年的猫新鲜冷冻血浆（fFFP）中凝血因子 I（纤维蛋白原）、II、V、VII、VIII、IX、X、XI 和 XII 的浓度和稳定性。
方法
从 55 只来自室内健康献血猫的新鲜全血供体中采集了 55 份 fFFP。其中 21 份储存<2 周（T0），34 份储存 1 年（T1）。储存完成后，使用改良的一期激活部分凝血活酶或凝血酶原时间测定法检测 II、V、VII、VIII、IX、X、XI 和 XII 特定凝血因子的活性。使用 Clauss 法测定纤维蛋白原。
结果
与 T0 相比，T1 时 II（T0：101.94%±19.06%；T1：73.23%±39.06% [ = 0.001]）、VII（T0：102.78%±24.69%；T1：60.08%±38.17% [ = 0.001]）、VIII（T0：77.52%±30.39%；T1：50.32%±23.8% [ = 0.001]）、XI（T0：88.76%±22.73%；T1：66.28%±22.2% [ = 0.001]）和 XII（T0：89.50%±21.85%；T1：55.46%±23.18% [ <0.001]）时的活性显著降低。因子 IX（T0：84.86%±29.35%；T1：71.37%±22.23% [ = 0.064]）和 X（T0：96.24%±25.1%；T1：83.91%±49.54% [ = 0.236]）无显著差异。出乎意料的是，因子 V（T0：71.94%±24.14%；T1：97.89%±62.33%； = 0.046）活性显著增加。T1 时纤维蛋白原为 2.76±1.09 g/l。VIII、XII 和 VII 是储存 1 年后活性最低的凝血因子。
结论和相关性
尽管储存后大多数凝血因子的活性降低，但 1 年的 fFFP在体外仍具有止血活性。VII 和 VIII 是最适合用于 fFFP 质量控制评估的因子。需要大约 13-20ml/kg 的 fFFP 来给予至少 10IU/kg 的凝血因子活性。
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