FCT, Universidade do Algarve, 8005-139 Faro, Portugal; CCMar, Universidade do Algarve, 8005-139 Faro, Portugal.
CCMar, Universidade do Algarve, 8005-139 Faro, Portugal; ISE, Universidade do Algarve, 8005-139 Faro, Portugal.
J Inorg Biochem. 2022 Nov;236:111952. doi: 10.1016/j.jinorgbio.2022.111952. Epub 2022 Jul 31.
Plasma membrane calcium ATPases (PMCA) and sarco(endo) reticulum calcium ATPases (SERCA) are key proteins in the maintenance of calcium homeostasis. Herein, we compare for the first time the inhibition of SERCA and PMCA calcium pumps by several polyoxotungstates (POTs), namely by Wells-Dawson phosphotungstate anions [PWO] (intact, {PW}), [PWO] (monolacunary, {PW}), [PWO] (trilacunary, {PW}), [HPWO] (hexalacunary, {PW}), [HPWVO] (trivanadium-substituted, {PWV}) and by Preyssler-type anion [NaPWO] ({PW}). The speciation in the solutions of tested POTs was investigated by P and V NMR spectroscopy. The tested POTs inhibited SERCA Ca-ATPase activity, whereby the Preyssler POT showed the strongest effect, with an IC value of 0.37 μM. For {PW} and {PWV} higher IC values were determined: 0.72 and 0.95 μM, respectively. The studied POTs showed to be more potent inhibitors of PMCA Ca-ATPase activity, with lower IC values for {PW}, {PW} and {PWV}.
质膜钙 ATP 酶 (PMCA) 和肌浆网/内质网钙 ATP 酶 (SERCA) 是维持钙稳态的关键蛋白。本文首次比较了几种多金属氧酸盐 (POTs) 对 SERCA 和 PMCA 钙泵的抑制作用，即 Wells-Dawson 磷钨酸盐阴离子 [PWO]（完整的，{PW}）、[PWO]（单缺位的，{PW}）、[PWO]（三缺位的，{PW}）、[HPWO]（六缺位的，{PW}）、[HPWVO]（钒取代的，{PWV}）和 Preyssler 型阴离子 [NaPWO] ({PW})。通过 P 和 V NMR 光谱研究了测试 POTs 溶液中的形态。测试的 POTs 抑制了 SERCA Ca-ATPase 活性，其中 Preyssler POT 表现出最强的抑制作用，IC 值为 0.37 μM。对于 {PW} 和 {PWV}，确定了更高的 IC 值：0.72 和 0.95 μM。研究的 POTs 对 PMCA Ca-ATPase 活性表现出更强的抑制作用，{PW}、{PW}和 {PWV} 的 IC 值较低。
