Lin Hengching, Toung Ooi Peck, Weng Hui-Xin, Hsu Peichuan, Chang Ming-Huang, Lai Jyhmirn
Department of Veterinary Medicine, College of Veterinary Medicine, National Chiayi University, 60004 Chiayi City, Taiwan.
Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia.
Res Vet Sci. 2022 Dec 10;151:184-188. doi: 10.1016/j.rvsc.2022.08.017. Epub 2022 Aug 25.
Except for Mycoplasma capricolum subsp. capripneumoniae, which only causes pneumonia, Mycoplasma species can cause otitis, arthritis, septicemia, mastitis, pneumonia and encephalitis in goats. After a mycoplasma outbreak was recorded in Taiwan in 2016, an increasing number of mycoplasma infection cases have been observed. It is important to understand how many Mycoplasma species are currently prevalent in Taiwan. In this study, 57-61 bulk milk samples were collected from dairy goats in Taiwan every year from 2017 to 2020 to identify the presence of mycoplasma DNA fragments by polymerase chain reaction (PCR). The mycoplasma species were identified based on colony characteristics, PCR, and DNA sequences. In total, 39 out of 236 samples (16.52%) tested mycoplasma positive. The annual mycoplasma prevalence rates from 2017 to 2020 were 24.59% (15/61), 8.20% (5/61), 17.54% (10/57) and 15.79% (9/57), respectively. Mycoplasma mycoides subsp. capri (n = 19), Mycoplasma putrefaciens (n = 10), Mycoplasma ovipneumoniae (n = 5) and Mycoplasma bovis (n = 5) were present in Taiwan. No M. capricolum subsp. capripneumoniae was detected. Except for the large drop in 2018, the prevalence of mycoplasma infection declined to a consistent level for the final two years of the study. However, the seasonal pattern is still worth investigating. This is the first survey of mycoplasma infection in goats, and we believe that a larger survey is still needed to understand the pathogenicity and diversity of these mycoplasma isolates.
除了仅引起肺炎的山羊支原体山羊肺炎亚种外，支原体属可导致山羊发生中耳炎、关节炎、败血症、乳腺炎、肺炎和脑炎。2016年台湾记录到支原体疫情爆发后，观察到的支原体感染病例数量不断增加。了解台湾目前流行的支原体种类数量很重要。在本研究中，2017年至2020年每年从台湾奶山羊中采集57 - 61份散装乳样，通过聚合酶链反应（PCR）鉴定支原体DNA片段的存在。根据菌落特征、PCR和DNA序列鉴定支原体种类。在总共236份样本中，有39份（16.52%）检测出支原体呈阳性。2017年至2020年的年度支原体流行率分别为24.59%（15/61）、8.20%（5/61）、17.54%（10/57）和15.79%（9/57）。台湾存在丝状支原体山羊亚种（n = 19）、腐败支原体（n = 10）、绵羊肺炎支原体（n = 5）和牛支原体（n = 5）。未检测到山羊支原体山羊肺炎亚种。除2018年大幅下降外，在研究的最后两年，支原体感染的流行率降至一个稳定水平。然而，季节性模式仍值得研究。这是首次对山羊支原体感染进行调查，我们认为仍需要进行更大规模的调查，以了解这些支原体分离株的致病性和多样性。
