Department of Biological Sciences, Birla Institute of Technology & Sciences Pilani, Pilani, Rajasthan, India, 333031.
Department of Biological Sciences, Birla Institute of Technology & Sciences Pilani, Pilani, Rajasthan, India, 333031.
Talanta. 2023 Jan 15;252:123843. doi: 10.1016/j.talanta.2022.123843. Epub 2022 Aug 17.
A fluorescent graphene oxide based aptasensing platform was developed for the detection of acephate. The aptamers specific to the acephate were screened through GO-SELEX (Graphene Oxide - Systematic Evolution of Ligands by EXponential enrichment) method for six rounds. The screened aptamers were analyzed for their binding affinity and specificity by using fluorescence-based assay. The aptamer AAPT3 that demonstrated highest affinity (K = 9 ± 1 nM) and an excellent selectivity, was employed in the development of fluorescent aptasensor. Under optimal conditions, the aptasensor showed low limit of detection (4 ng mL) and a wide dynamic linear range (5-80 ng mL). The aptasensor was also validated against water samples spiked with acephate, which showed fluorescence recovery from 94 to 107% and coefficient of variation 1-5%. These results indicate that the developed aptasensor can be used for sensitive, selective, and accurate detection of acephate in various samples.
一种基于荧光氧化石墨烯的适体传感平台被开发用于检测乙酰甲胺磷。通过 GO-SELEX（氧化石墨烯-指数富集配体系统进化）方法筛选出对乙酰甲胺磷具有特异性的适体，并通过荧光测定法分析其结合亲和力和特异性。筛选出的适体 AAPT3 表现出最高的亲和力（K = 9 ± 1 nM）和优异的选择性，被用于荧光适体传感器的开发。在最佳条件下，该适体传感器具有低检测限（4 ng mL）和宽动态线性范围（5-80 ng mL）。该适体传感器还针对含有乙酰甲胺磷的水样进行了验证，荧光恢复率为 94%至 107%，变异系数为 1-5%。这些结果表明，所开发的适体传感器可用于各种样品中乙酰甲胺磷的灵敏、选择性和准确检测。
