Lee Tai-Fen, Lin Kuan-Yin, Chang Sui-Yuan, Huang Yu-Tsung, Hsueh Po-Ren
Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan.
Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.
J Microbiol Immunol Infect. 2023 Feb;56(1):104-110. doi: 10.1016/j.jmii.2022.08.009. Epub 2022 Aug 18.
This study aimed to investigate the etiologies of sexually transmitted infections (STIs) among men who have sex with men (MSM) in Taiwan.
Two commercial assays, the BD MAX Chlamydia trachomatis (CT), Neisseria gonorrhoeae (GC), and Trichomonas vaginalis (TV) panel and the Allplex™ STI Essential assay (CT, GC, Mycoplasma genitalium [MG], Mycoplasma hominis [MH], Ureaplasma urealyticum [UU], Ureaplasma parvum [UP], and TV) were evaluated. During the first stage, urine and rectal swab samples from 168 patients were evaluated using the BD MAX assay, and the multiplex RT-PCR Allplex™ STI Essential assay was applied only to the patients with positive results on the BD MAX asay (n = 49). During the second stage, urine and rectal swab samples from 90 patients were evaluated using the BD MAX assay and the Allplex™ qPCR.
The Allplex qPCR identified all CT, missed one and additionally one TV from the positive samples (n = 49) by the BD MAX assay in the first stage. At the second stage, both commercial assays showed similar detection rate of CT, NG or CT/NG coinfection (11.1%, 1.1% and 4.4% by the BD MAX assay; 10.0%, 1.1% and 2.2% by the Allplex qPCR). The positivity rates of MG, MH, and UU by the Allplex qPCR were 4.4%, 2.2%, and 12.2%, respectively, for urine samples and 10%, 13.3%, and 22.2%, respectively, for anal swab samples.
High rates of STI-associated etiologies were observed in MSM. The positive rates were higher in rectal swabs than in urine samples.
本研究旨在调查台湾男男性行为者（MSM）中性传播感染（STI）的病因。
评估了两种商业检测方法，即BD MAX沙眼衣原体（CT）、淋病奈瑟菌（GC）和阴道毛滴虫（TV）检测板以及Allplex™ STI Essential检测方法（CT、GC、生殖支原体[MG]、人型支原体[MH]、解脲脲原体[UU]、微小脲原体[UP]和TV）。在第一阶段，使用BD MAX检测方法对168例患者的尿液和直肠拭子样本进行评估，多重RT-PCR Allplex™ STI Essential检测方法仅应用于BD MAX检测结果为阳性的患者（n = 49）。在第二阶段，使用BD MAX检测方法和Allplex™ qPCR对90例患者的尿液和直肠拭子样本进行评估。
在第一阶段，Allplex qPCR检测出了BD MAX检测方法在阳性样本（n = 49）中鉴定出的所有CT，漏检了1例TV，且额外检出1例TV。在第二阶段，两种商业检测方法对CT、NG或CT/NG合并感染的检测率相似（BD MAX检测方法分别为11.1%、1.1%和4.4%；Allplex qPCR分别为10.0%、1.1%和2.2%）。Allplex qPCR对尿液样本中MG、MH和UU阳性率分别为4.4%、2.2%和12.2%，对肛门拭子样本中阳性率分别为10%、13.3%和22.2%。
在男男性行为者中观察到性传播感染相关病因的高发生率。直肠拭子中的阳性率高于尿液样本。
