Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, 37077, Göttingen, Germany.
Department of Applied Bioinformatics, Göttingen Center for Molecular Biosciences (GZMB) and Campus Institute Data Science (CIDAS), Institute for Microbiology and Genetics, University of Göttingen, 37077, Göttingen, Germany.
Plant J. 2022 Oct;112(2):518-534. doi: 10.1111/tpj.15964. Epub 2022 Sep 16.
There are numerous examples of plant organs or developmental stages that are desiccation-tolerant and can withstand extended periods of severe water loss. One prime example are seeds and pollen of many spermatophytes. However, in some plants, also vegetative organs can be desiccation-tolerant. One example are the tubers of yellow nutsedge (Cyperus esculentus), which also store large amounts of lipids similar to seeds. Interestingly, the closest known relative, purple nutsedge (Cyperus rotundus), generates tubers that do not accumulate oil and are not desiccation-tolerant. We generated nanoLC-MS/MS-based proteomes of yellow nutsedge in five replicates of four stages of tuber development and compared them to the proteomes of roots and leaves, yielding 2257 distinct protein groups. Our data reveal a striking upregulation of hallmark proteins of seeds in the tubers. A deeper comparison to the tuber proteome of the close relative purple nutsedge (C. rotundus) and a previously published proteome of Arabidopsis seeds and seedlings indicates that indeed a seed-like proteome was found in yellow but not purple nutsedge. This was further supported by an analysis of the proteome of a lipid droplet-enriched fraction of yellow nutsedge, which also displayed seed-like characteristics. One reason for the differences between the two nutsedge species might be the expression of certain transcription factors homologous to ABSCISIC ACID INSENSITIVE3, WRINKLED1, and LEAFY COTYLEDON1 that drive gene expression in Arabidopsis seed embryos.
有许多植物器官或发育阶段具有耐旱性，可以承受长时间的严重水分流失。一个主要的例子是许多种子植物的种子和花粉。然而，在一些植物中，营养器官也可以耐旱。一个例子是黄姜（Cyperus esculentus）的块茎，它也储存大量类似于种子的脂质。有趣的是，已知最接近的亲缘植物，紫姜（Cyperus rotundus），其块茎不积累油脂且不耐干旱。我们生成了五个重复样本的黄姜块茎发育四个阶段的基于 nanoLC-MS/MS 的蛋白质组，并将其与根和叶的蛋白质组进行了比较，得到了 2257 个独特的蛋白质组。我们的数据显示，块茎中标志性的种子蛋白显著上调。与近亲紫姜（C. rotundus）的块茎蛋白质组以及先前发表的拟南芥种子和幼苗的蛋白质组进行更深入的比较表明，在黄姜中确实发现了类似于种子的蛋白质组，但在紫姜中没有。这进一步得到了对富含黄姜脂质体的蛋白质组的分析的支持，该脂质体也显示出种子样的特征。这两个姜属物种之间差异的一个原因可能是某些转录因子的表达，这些转录因子与拟南芥种子胚胎中的脱落酸不敏感 3（ABSCISIC ACID INSENSITIVE3）、卷曲（WRINKLED1）和胚乳（LEAFY COTYLEDON1）同源，驱动基因表达。
