Kushwah Ashutosh, Bhatia Dharminder, Singh Gurpreet, Singh Inderjit, Vij Suruchi, Bindra Shayla, Siddique Kadambot H M, Nayyar Harsh, Singh Sarvjeet
Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab India 141004.
Regional Research Station, Punjab Agricultural University, Faridkot, India.
Physiol Mol Biol Plants. 2022 Jul;28(7):1437-1452. doi: 10.1007/s12298-022-01218-z. Epub 2022 Aug 13.
Drought is a major abiotic stress that drastically reduces chickpea yields. The present study was aimed to identify drought-responsive traits in chickpea by screening a recombinant inbred line population derived from an inter-specific cross between drought cultivar of GPF2 ( L.) and drought sensitive accession of ILWC292 (), at two locations in India. Twenty-one traits, including twelve morphological and physiological traits and nine root-related traits were measured under rainfed (drought-stress) and irrigated conditions (no-stress). High genotypic variation was observed among RILs for yield and root traits indicated that selection in these germplasms would be useful in achieving genetic progress. Both correlation and principal component analysis revealed that plant height, number of pods per plant, biomass, 100-seed weight, harvest index, membrane permeability index, and relative leaf water content were significantly correlated with yield under both irrigated and drought stress environments. Root length had significant positive correlations with all root-related traits except root length density in drought-stressed plants. Path analysis and multiple and stepwise regression analyses showed that number of pods per plant, biomass, and harvest index were major contributors to yield under drought stress conditions. Thus, a holistic approach across these analyses identified number of pods per plant, biomass, harvest index, and root length as key traits for improving chickpea yield through indirect selection for developing drought-tolerant cultivars. Overall, on the basis of yield components morphological and root traits, a total of 15 promising RILs were identified for their use in chickpea breeding programs for developing drought tolerant cultivars.
The online version contains supplementary material available at 10.1007/s12298-022-01218-z.
干旱是一种严重的非生物胁迫，会大幅降低鹰嘴豆产量。本研究旨在通过在印度的两个地点筛选由耐旱品种GPF2（L.）与干旱敏感材料ILWC292（）种间杂交衍生的重组自交系群体，来鉴定鹰嘴豆中的干旱响应性状。在雨养（干旱胁迫）和灌溉条件（无胁迫）下测量了21个性状，包括12个形态和生理性状以及9个根系相关性状。在重组自交系中观察到产量和根系性状存在高度基因型变异，这表明在这些种质中进行选择将有助于实现遗传进展。相关性分析和主成分分析均表明，在灌溉和干旱胁迫环境下，株高、单株荚数、生物量、百粒重、收获指数、膜透性指数和相对叶片含水量均与产量显著相关。在干旱胁迫植株中，根长与除根长密度外的所有根系相关性状均呈显著正相关。通径分析以及多元和逐步回归分析表明，在干旱胁迫条件下，单株荚数、生物量和收获指数是产量的主要贡献因素。因此，通过这些分析的整体方法确定，单株荚数、生物量、收获指数和根长是通过间接选择培育耐旱品种来提高鹰嘴豆产量的关键性状。总体而言，基于产量构成因素、形态和根系性状，共鉴定出15个有前景的重组自交系，可用于鹰嘴豆育种计划以培育耐旱品种。
在线版本包含可在10.1007/s12298-022-01218-z获取的补充材料。
