Zhang Jinqing, Ma Huiling
College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S. Center for Grazingland Ecosystem Sustainability, Lanzhou, 730070 Gansu China.
Physiol Mol Biol Plants. 2022 Jul;28(7):1359-1374. doi: 10.1007/s12298-022-01216-1. Epub 2022 Aug 22.
MADS-box genes play vital roles in multiple biological processes of plants growth and development, especially inflorescence development. In the present study, a comprehensive investigation into the identification and classification of MADS-box genes in Kentucky bluegrass () has not been reported. Here, based on the transcriptome of inflorescence, we identified 44 PpMADS-box genes, and gave an overview of the physicochemical properties, phylogeny, protein structures, and potential functions of the proteins encoded by these genes through various bioinformatics software for the first time. Analysis of physicochemical properties revealed that most PpMADS-box were alkaline proteins and possessed similar conserved motifs. Additionally, it was demonstrated that 33 PpMADS-box proteins without signal peptide, leading peptide, transmembrane structure and located in the nucleus were not transported or secreted, so directly played transcriptional regulatory roles in the nucleus. Then, peptide sequences BLAST search and analysis of phylogenetic relationships with MADS-box proteins of , , and were performed. It was found that 44 PpMADS-box proteins were separated into 33 MIKC-type (3 BS, 1 AGL17, 8 AP3/P2, 3 AP1, 5 SEP, 6 SOC and 7 AG genes, respectvely) and 11 type I-type, which include 7 Mγ and 4 Mα. Furthermore, the relative expression levels of the selected 12 genes (, , , , , , , , , , and ) at the booting stage, pre-anthesis, anthesis, post-anthesis, and seed filling stage of inflorescences, as well as leaves and roots of the corresponding stages of inflorescences were analyzed, showing that most PpMADS-box genes were highly expressed mainly in young leaves and later inflorescences, and had complex patters in roots. Morever, except for being highly expressed in the leaves, others were significantly highly expressed in inflorescence and/ or roots, demonstrating PpMADS-box genes also regulate leaves and roots development in plant. This study provides valuable insights into the MADS-box family genes in Kentucky bluegrass and its potential functional characteristics, expression pattern, and evolution in floral organogenesis and even reproduction development. @media print { .ms-editor-squiggler { display:none !important; } } .ms-editor-squiggler { all: initial; display: block !important; height: 0px !important; width: 0px !important; }.
The online version contains supplementary material available at 10.1007/s12298-022-01216-1.
MADS盒基因在植物生长发育的多个生物学过程中发挥着至关重要的作用，尤其是在花序发育方面。在本研究中，尚未见对草地早熟禾中MADS盒基因的鉴定和分类进行全面调查的报道。在此，基于花序转录组，我们首次鉴定出44个PpMADS盒基因，并通过各种生物信息学软件对这些基因编码蛋白的理化性质、系统发育、蛋白质结构及潜在功能进行了概述。理化性质分析表明，大多数PpMADS盒蛋白为碱性蛋白，并具有相似的保守基序。此外，结果表明33个PpMADS盒蛋白无信号肽、前导肽和跨膜结构，且位于细胞核中，不进行转运或分泌，因此直接在细胞核中发挥转录调控作用。然后，进行了肽序列BLAST搜索以及与小麦、水稻和拟南芥的MADS盒蛋白的系统发育关系分析。结果发现，44个PpMADS盒蛋白被分为33个MIKC型（分别为3个BS、1个AGL17、8个AP3/P2、3个AP1、5个SEP、6个SOC和7个AG基因）和11个I型，其中包括7个Mγ和4个Mα。此外，分析了所选12个基因（PpMADS1、PpMADS2、PpMADS3、PpMADS4、PpMADS5、PpMADS6、PpMADS7、PpMADS8、PpMADS9、PpMADS10、PpMADS11和PpMADS12）在花序的孕穗期、开花前、开花期、开花后和灌浆期以及相应花序阶段的叶片和根中的相对表达水平，结果表明大多数PpMADS盒基因主要在幼叶和后期花序中高表达，在根中具有复杂的表达模式。此外，除PpMADS12在叶片中高表达外，其他基因在花序和/或根中显著高表达，表明PpMADS盒基因也调控植物叶片和根的发育。本研究为草地早熟禾中的MADS盒家族基因及其潜在功能特征、表达模式以及在花器官发生甚至生殖发育中的进化提供了有价值的见解。@media print {.ms-editor-squiggler { display:none !important; } }.ms-editor-squiggler { all: initial; display: block !important; height: 0px !important; width: 0px !important; }.
在线版本包含可在10.1007/s12298-022-01216-1获取的补充材料。
