Laboratorio de Inmunología, Unidad de Morfología y Función, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, Estado de México, Mexico.
Secretaría de Salud de Veracruz, Servicios de Salud de Veracruz, SESVER, Xalapa Veracruz, Mexico.
Front Immunol. 2022 Aug 16;13:946770. doi: 10.3389/fimmu.2022.946770. eCollection 2022.
The current pandemic generated by SARS-CoV-2 has led to mass vaccination with different biologics that have shown wide variations among human populations according to the origin and formulation of the vaccine. Studies evaluating the response in individuals with a natural infection before vaccination have been limited to antibody titer analysis and evaluating a few humoral and cellular response markers, showing a more rapid and intense humoral response than individuals without prior infection. However, the basis of these differences has not been explored in depth. In the present work, we analyzed a group of pro and anti-inflammatory cytokines, antibody titers, and cell populations in peripheral blood of individuals with previous SARS-CoV-2 infection using BNT162b2 biologic. Our results suggest that higher antibody concentration in individuals with an earlier disease could be generated by higher production of plasma cells to the detriment of the presence of memory B cells in the bloodstream, which could be related to the high baseline expression of cytokines (IL-6 and IL-10) before vaccination.
当前由 SARS-CoV-2 引起的大流行导致了针对不同生物制剂的大规模疫苗接种，根据疫苗的来源和配方，这些生物制剂在人类群体中显示出广泛的差异。评估个体在接种疫苗前自然感染后的反应的研究仅限于抗体滴度分析和评估少数体液和细胞反应标志物，显示出比没有先前感染的个体更快和更强烈的体液反应。然而，这些差异的基础尚未深入探讨。在本工作中，我们使用 BNT162b2 生物制剂分析了一组先前感染过 SARS-CoV-2 的个体的外周血中的促炎和抗炎细胞因子、抗体滴度和细胞群体。我们的结果表明，早期疾病患者体内更高的抗体浓度可能是由于浆细胞产生增加而导致的，这对血液中记忆 B 细胞的存在不利，这可能与接种前细胞因子（IL-6 和 IL-10）的高基线表达有关。
