Centro de Investigación en Ciencias de la Salud (CICSA), FCS, Universidad Anáhuac México Campus Norte, Huixquilucan, Edo. de México, Mexico.
CNS Neurosci Ther. 2022 Dec;28(12):1986-1992. doi: 10.1111/cns.13951. Epub 2022 Sep 2.
The mechanism underlying the memory improvement induced by prebiotic and probiotic supplementation remains unclear. Glucagon-like peptide type 1 (GLP-1) could play an important role since it is induced by prebiotics and enhances memory and learning.
We correlated the levels of GLP-1 with spatial memory in senile animals to determine its role in memory improvement after prebiotic and probiotic supplementation.
Senile rats were randomly assigned to four groups: (1) water (control); (2) Enterococcus faecium (probiotic); (3) agave inulin (prebiotic); and (4) E. faecium + agave inulin (symbiotic). Each supplement was administered by an orogastric cannula for 5 weeks. In the fifth week, spatial memory was assessed using the Morris Water Maze test (MWM). We extracted the hippocampus, intestine, and serum. GLP-1 levels were quantified by enzyme-linked immunosorbent assay.
A significant decrease in escape latency time in the MWM was observed in all groups treated with supplements. The symbiotic group achieved the highest reduction (15.13 s ± 6.40) (p < 0.01). We did not find a significant increase in GLP-1 levels nor a direct correlation of its levels with spatial memory improvement (p > 0.05).
Prebiotic and probiotic supplementation improved spatial memory in senile animals. However, this beneficial effect did not correlate with GLP-1 levels.
益生菌和益生元补充剂诱导记忆改善的机制尚不清楚。胰高血糖素样肽 1 (GLP-1) 可能发挥重要作用，因为它可被益生元诱导，增强记忆和学习能力。
我们将 GLP-1 水平与老年动物的空间记忆相关联，以确定其在益生菌和益生元补充后改善记忆中的作用。
将老年大鼠随机分为四组：(1)水（对照）；(2)粪肠球菌（益生菌）；(3)龙舌兰花蜜菊苣纤维（益生元）；(4)粪肠球菌+龙舌兰花蜜菊苣纤维（共生）。每个补充剂通过经口胃管给药 5 周。在第 5 周，使用 Morris 水迷宫测试（MWM）评估空间记忆。我们提取海马体、肠道和血清。通过酶联免疫吸附试验定量 GLP-1 水平。
所有接受补充剂治疗的组在 MWM 中的逃避潜伏期时间均显著缩短。共生组的降低幅度最大（15.13s±6.40）（p<0.01）。我们没有发现 GLP-1 水平显著增加，也没有发现其水平与空间记忆改善之间存在直接相关性（p>0.05）。
益生菌和益生元补充剂改善了老年动物的空间记忆。然而，这种有益作用与 GLP-1 水平无关。
