人源肌球蛋白重链 5 同源二聚体的冷冻电镜结构。

Cryo-EM structure of human MG53 homodimer.

机构信息

State Key Laboratory of Membrane Biology, College of Future Technology, Institute of Molecular Medicine, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Peking University, Beijing 100871, China.

National Biomedical Imaging Center, Peking University, Beijing 100871, China.

出版信息

Biochem J. 2022 Sep 16;479(17):1909-1916. doi: 10.1042/BCJ20220385.

DOI:10.1042/BCJ20220385

PMID:36053137

Abstract

MG53 is a tripartite motif (TRIM) family E3 ligase and plays important biological functions. Here we present the cryo-EM structure of human MG53, showing that MG53 is a homodimer consisting of a 'body' and two 'wings'. Intermolecular interactions are mainly distributed in the 'body' which is relatively stable, while two 'wings' are more dynamic. The overall architecture of MG53 is distinct from those of TRIM20 and TRIM25, illustrating the broad structural diversity of this protein family.

摘要

MG53 是一个三联基序（TRIM）家族 E3 连接酶，发挥着重要的生物学功能。在这里，我们呈现了人类 MG53 的冷冻电镜结构，表明 MG53 是一个由“主体”和两个“翅膀”组成的同源二聚体。分子间相互作用主要分布在相对稳定的“主体”中，而两个“翅膀”则更加动态。MG53 的整体结构与 TRIM20 和 TRIM25 的不同，说明了这个蛋白质家族具有广泛的结构多样性。