Goh Yeongchang, Kim Jongwoo, Park Hye Sun, Jung Taeyoung, Hong Kwan Soo, Nam Sang Hwan, Suh Yung Doug, Lee Kang Taek
Department of Chemistry, Gwangju Institute of Science and Technology (GIST), Gwangju, South Korea.
Laboratory for Advanced Molecular Probing (LAMP), Korea Research Institute of Chemical Technology (KRICT), Daejeon, South Korea.
Nanoscale. 2022 Oct 6;14(38):14008-14013. doi: 10.1039/d2nr01999j.
Cell-cell communication is important for cellular differentiation, organ function, and immune responses. In intercellular communication, the extracellular vesicles (EVs) play a significant role in delivering the cargo molecules such as genes, proteins, and enzymes, to regulate and control the ability of the recipient cells. In this study, the observation of intercellular cargo transfer dual-colour imaging using upconverting nanoparticles (UCNPs) has been demonstrated. Using this technique, the intercellular transport contact-dependent and contact-independent signaling in live HeLa cells was clearly visualized with real-time, long-term single-vesicle tracking. Furthermore, it was demonstrated that the endocytosed UCNPs can be transmitted with the encapsulation of EVs labelled with fluorescent proteins.
细胞间通讯对于细胞分化、器官功能和免疫反应至关重要。在细胞间通讯中，细胞外囊泡（EVs）在传递基因、蛋白质和酶等货物分子以调节和控制受体细胞的能力方面发挥着重要作用。在本研究中，已经证明了使用上转换纳米颗粒（UCNPs）进行细胞间货物转移双色成像的观察。利用该技术，通过实时、长期的单囊泡追踪，可以清晰地观察到活HeLa细胞中细胞间运输的接触依赖性和接触非依赖性信号传导。此外，还证明了内吞的UCNPs可以与标记有荧光蛋白的EVs封装一起进行传递。
