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通过协同磷酸化/超声改善鸭蛋蛋清的凝胶性能:凝胶性能、晶体结构和蛋白质结构。

Improving the gel properties of duck egg white by synergetic phosphorylation/ultrasound: Gel properties, crystalline structures, and protein structure.

机构信息

Jiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang 330045, China; Engineering Research Center of Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, China; Agricultural Products Processing and Quality Control Engineering Laboratory of Jiangxi, Jiangxi Agricultural University, Nanchang 330045, China.

Engineering Research Center of Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, China.

出版信息

Ultrason Sonochem. 2022 Sep;89:106149. doi: 10.1016/j.ultsonch.2022.106149. Epub 2022 Aug 29.

Abstract

To improve the gel properties of duck egg white gel and increase the industrial value of duck egg white, the mechanisms of ultrasound and synergetic phosphorylation/ultrasound treatments were examined in this study. It was found that as the ultrasound power increased, the surface hydrophobicity, hardness, and cohesiveness of the gel system increased, and the ζ-potential and water mobility decreased. Of the two treatments, phosphorylation/ultrasound had the strongest impact on the conformation and crystallinity of the gel system and promoted the formation of high molecular polymers. Both gel systems displayed enhanced compactness, stability, and gel strength because of the enhanced protein-protein interactions via hydrogen bonds and protein aggregation, and increased the content of intramolecular β-sheets following ultrasound treatment, and synergetic phosphorylation/ultrasound further improved the stability, water binding and gel properties. This experiment showed that ultrasound and, particularly, phosphorylation/ultrasound are effective methods to improve the gel properties of duck egg white. This study enhanced our understanding of the interactions of sodium pyrophosphate and egg white under ultrasound treatment, and promote the potential application of sodium pyrophosphate and ultrasound treatment of novel food products.

摘要

为了改善鸭蛋蛋清凝胶的凝胶性能,提高鸭蛋蛋清的工业价值,本研究考察了超声和协同磷酸化/超声处理的机制。结果发现,随着超声功率的增加,凝胶体系的表面疏水性、硬度和内聚性增加,ζ-电位和水分流动性降低。在这两种处理方法中,磷酸化/超声对凝胶体系的构象和结晶度影响最大,促进了高分子聚合物的形成。两种凝胶体系的稳定性、凝胶强度和紧凑性都得到了增强,这是由于氢键和蛋白质聚集增强了蛋白质-蛋白质相互作用,并且超声处理后增加了分子内β-折叠的含量,协同磷酸化/超声进一步提高了稳定性、保水性和凝胶性能。该实验表明,超声,特别是磷酸化/超声是改善鸭蛋蛋清凝胶性能的有效方法。本研究增强了我们对焦磷酸钠和蛋清在超声处理下相互作用的理解,并促进了焦磷酸钠和超声处理在新型食品中的潜在应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ee1/9449846/be88f6c3883f/gr1.jpg

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