Center of Biological and Health Sciences, State University of Western Paraná, Cascavel, Paraná, Brazil.
Center of Biological and Health Sciences, State University of Western Paraná, Cascavel, Paraná, Brazil.
J Manipulative Physiol Ther. 2022 May;45(4):248-260. doi: 10.1016/j.jmpt.2022.07.001. Epub 2022 Aug 30.
The purpose of this study was to analyze the systemic effects of stair climbing exercise, low-level laser therapy (LLLT), and the association of both treatments on pelvic member functionality and ankle joint histomorphometric aspects of Wistar rats submitted to experimental rheumatoid arthritis (RA) protocol.
Male Wistar rats were randomly distributed into the following 8 groups: control; control LLLT; control exercise; control LLLT and exercise; arthritis group; arthritis LLLT; arthritis exercise; and arthritis LLLT and exercise, with n = 8 for functional and n = 5 for histomorphometrical tests. The experimental RA was induced by complete Freund adjuvant injection in the knee joint cavity. Functionality was evaluated by proprioception and motor function using Sciatic Functional Index and maximum angle reached at an inclined plane. Histomorphometrical aspects were evaluated in the ankle joint after histological routine.
The arthritis LLLT and exercise group had positive effects in Sciatic Functional Index (F [3.96] = 11.3, P < .001) and in inclined plane (F [3.4] = 36.1, P < .001). The arthritis exercise group presented a greater number of chondrocytes in the tibia (Wald [1; 6605.6] = 25.2, P < .001) and talus (Wald [1; 15958.6] = 19.8, P = .006) in relation to the other groups. The arthritis group morphology showed significant degenerative lesions as subintima with angiogenesis, inflammatory cells, flocculated articular cartilage, chondrocytes disorganization and pannus. Even with the higher chondrocytes number, the arthritis exercise group had morphological characteristics more similar to the control group.
Low-level laser therapy and exercise restored functionality, and exercise restored morphological aspects of tissues in experimentally induced RA in rats.
本研究旨在分析爬楼梯运动、低水平激光疗法（LLLT）的全身效应，以及两者联合治疗对实验性类风湿关节炎（RA）Wistar 大鼠骨盆成员功能和踝关节组织形态学的影响。
雄性 Wistar 大鼠随机分为以下 8 组：对照组；对照组 LLLT；对照组运动；对照组 LLLT 和运动；关节炎组；关节炎 LLLT；关节炎运动；关节炎 LLLT 和运动，每组 n=8 进行功能测试，n=5 进行组织形态学测试。通过完全弗氏佐剂向膝关节腔内注射诱导实验性 RA。使用坐骨神经功能指数和在斜面上达到的最大角度评估运动功能和本体感受功能。在踝关节进行常规组织学评估后，对组织形态学进行评估。
关节炎 LLLT 和运动组在坐骨神经功能指数（F [3.96] = 11.3，P <.001）和斜面（F [3.4] = 36.1，P <.001）方面有积极影响。与其他组相比，关节炎运动组胫骨（Wald [1；6605.6] = 25.2，P <.001）和距骨（Wald [1；15958.6] = 19.8，P =.006）的软骨细胞数量更多。关节炎组的形态学表现出显著的退行性病变，包括亚内膜血管生成、炎症细胞、絮状关节软骨、软骨细胞排列紊乱和肉芽组织。即使软骨细胞数量增加，关节炎运动组的形态特征仍更类似于对照组。
低水平激光疗法和运动恢复了功能，运动恢复了实验性诱导的 RA 大鼠组织的形态学特征。
