Institute of Biological Sciences and Health, Federal University of Alagoas - UFAL, Lourival Melo Mota, s/n, Tabuleiro dos Martins, Maceió, AL, 57072-900, Brazil.
Department of Restorative Dentistry, Federal University of Paraíba - UFPB, João Pessoa, PB, Brazil.
Lasers Med Sci. 2020 Jul;35(5):1129-1139. doi: 10.1007/s10103-019-02933-y. Epub 2019 Dec 13.
This study aimed to investigate the anti-inflammatory effects of different dosage of low-level laser therapy (LLLT) in an experimental model of temporomandibular joint (TMJ) arthritis. One hundred male Wistar rats were used and divided into the following groups: CG, control group; AG, animals group with left TMJ arthritis induced by intra-articular injection of Complete Freund's adjuvant - CFA; LG5, LG10 and LG20 - animals with arthritis and treated with LLLT at doses 5, 10, and 20 J/cm, respectively. Morphological analysis was performed by TMJ histological sections stained with hematoxylin-eosin (HE), picrosirius (PSR), and toluidine blue (TB), as well as histomorphometric evaluation of cartilage, articular disc, and masticatory muscles. The amount of feed consumed within 3 weeks was evaluated, and biochemical analysis of TMJ tissues included measurement of sulfated glycosaminoglycans (GAGs), matrix metalloproteinases (MMPs) 2 and 9 zymography, and ELISA for cytokines IL-6, TNF-α, and IL-1β. Only the 20 J/cm dose promoted higher feed intake compared to AG. On the other hand, all LLLT doses promoted better organization of articular disc collagen fibers, greater number of proteoglycans in articular cartilage, increased area and diameter of left lateral pterygoid fibers, reduced latent and active MMP 9 and 2 activity, and lower IL-1β concentration compared to AG. Considering the study limitations, it was observed that LLLT treatments were effective in protecting and tissue cleansing joint structures, accelerating tissue repair, especially at lower doses.
本研究旨在探讨不同剂量低水平激光疗法（LLLT）在颞下颌关节（TMJ）关节炎实验模型中的抗炎作用。使用 100 只雄性 Wistar 大鼠，并将其分为以下几组：CG，对照组；AG，通过关节内注射完全弗氏佐剂-CFA 诱导左侧 TMJ 关节炎的动物组；LG5、LG10 和 LG20-关节炎动物，分别接受 5、10 和 20 J/cm 剂量的 LLLT 治疗。通过 TMJ 组织学切片用苏木精-伊红（HE）、苦味酸（PSR）和甲苯胺蓝（TB）染色进行形态学分析，以及软骨、关节盘和咀嚼肌的组织形态计量学评估。评估了 3 周内消耗的饲料量，TMJ 组织的生化分析包括测量硫酸化糖胺聚糖（GAGs）、基质金属蛋白酶（MMPs）2 和 9 酶谱和细胞因子 IL-6、TNF-α 和 IL-1β 的 ELISA。只有 20 J/cm 剂量与 AG 相比促进了更高的饲料摄入量。另一方面，所有 LLLT 剂量均促进了关节盘胶原纤维更好的组织化、关节软骨中更多的蛋白聚糖、左侧翼外肌纤维面积和直径增加、潜伏和活性 MMP 9 和 2 活性降低以及 IL-1β 浓度降低与 AG 相比。考虑到研究的局限性，观察到 LLLT 治疗在保护和清洁关节结构方面是有效的，特别是在较低剂量下，能加速组织修复。
