Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, School of Environment and Energy, South China University of Technology, Guangzhou, 510006, China.
Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, School of Environment and Energy, South China University of Technology, Guangzhou, 510006, China.
J Environ Manage. 2022 Oct 15;320:115891. doi: 10.1016/j.jenvman.2022.115891. Epub 2022 Aug 11.
Doxycycline hydrochloride (DCH) could be continuously removed by Bacillus thuringiensis S622 with the in-situ biogenic manganese oxide (BioMnOx) via oxidizing/regenerating. The DCH removal rate was significantly increased by 3.01-fold/1.47-fold at high/low Mn loaded via the integration of biological (intracellular/extracellular electron transfer (IET/EET)) and abiotic process (BioMnOx, Mn(III) and •OH). BioMnOx accelerated IET via activating coenzyme Q to enhance electrons transfer (ET) from complex I to complex III, and as an alternative electron acceptor for respiration and provide another electron transfer transmission channel. Additionally, EET was also accelerated by stimulating to secrete flavins, cytochrome c (c-Cyt) and flavin bounded with c-Cyt (Flavins & Cyts). To our best knowledge, this is the first report about the role of BioMnOx on IET/EET during antibiotic biodegradation. These results suggested that Bacillus thuringiensis S622 incorporated with BioMnOx could adopt an alternative strategy to enhance DCH degradation, which may be of biogeochemical and technological significance.
盐酸强力霉素（DCH）可通过苏云金芽孢杆菌 S622 原位生物生成的氧化锰（BioMnOx）氧化/再生而被连续去除。通过整合生物（细胞内/细胞外电子转移（IET/EET）和非生物过程（BioMnOx、Mn(III) 和 •OH），高/低 Mn 负载时 DCH 的去除率分别显著提高了 3.01 倍/1.47 倍。BioMnOx 通过激活辅酶 Q 来加速 IET，从而增强复合物 I 到复合物 III 的电子转移（ET），并作为呼吸的替代电子受体，提供另一个电子转移传输通道。此外，通过刺激黄素、细胞色素 c（c-Cyt）和 Flavins & Cyts 的分泌，也加速了 EET。据我们所知，这是关于 BioMnOx 在抗生素生物降解过程中对 IET/EET 的作用的首次报道。这些结果表明，苏云金芽孢杆菌 S622 与 BioMnOx 结合后可以采用替代策略来增强 DCH 的降解，这可能具有生物地球化学和技术意义。
