CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Key Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China.
Mar Pollut Bull. 2022 Oct;183:114058. doi: 10.1016/j.marpolbul.2022.114058. Epub 2022 Aug 31.
The dinoflagellate genus Alexandrium comprises most of the toxic bloom-forming species producing paralytic shellfish toxins (PSTs) in the sea. Recently, repeated paralytic shellfish poisoning episodes have been recorded in Qinhuangdao located at the west coast of the Bohai Sea. To elucidate the relationship between toxic Alexandrium blooms and the poisoning episodes, a year-round investigation was carried out in this region from July 2020 to July 2021. Two qPCR assays were used to detect A. catenella and A. pacificum, and LC-MS/MS was applied to analyze PSTs in phytoplankton and shellfish samples. The blooms of A. catenella and A. pacificum were found in April and July, respectively, and PST content in three bivalves exhibited notable increase following the bloom of A. catenella. The results revealed bloom dynamics of the two toxic Alexandrium species in the Bohai Sea for the first time, and further confirmed A. catenella as the causative agent of poisoning episodes.
甲藻属中的亚历山大藻是产生麻痹性贝类毒素（PSTs）的有毒赤潮生物的主要类群。最近，在位于渤海湾西岸的秦皇岛发生了多起麻痹性贝类中毒事件。为了阐明有毒亚历山大藻赤潮与中毒事件之间的关系，本研究于 2020 年 7 月至 2021 年 7 月对该地区进行了全年调查。使用两种 qPCR 检测方法检测了短凯伦藻和太平洋短沟藻，并用 LC-MS/MS 分析了浮游植物和贝类样品中的 PSTs。发现短凯伦藻和太平洋短沟藻的赤潮分别出现在 4 月和 7 月，并且在短凯伦藻赤潮之后，三种双壳贝类中的 PST 含量显著增加。结果首次揭示了渤海湾这两种有毒亚历山大藻的赤潮动态，并进一步证实了短凯伦藻是中毒事件的致病因子。
