Centre for Biotechnology, Maharshi Dayanand University, Rohtak 124001, India.
Centre for Biotechnology, Maharshi Dayanand University, Rohtak 124001, India; Dept. of Microbiology, Pt. B. D. Sharma University of Health Sciences, Rohtak 124001, India.
J Microbiol Methods. 2022 Oct;201:106563. doi: 10.1016/j.mimet.2022.106563. Epub 2022 Aug 31.
A real-time immuno-PCR assay was deliberated to detect mycobacterial mannophosphoinositides (PIMs). A dynamic range of PIMs (0.9 pg/mL-10 ng/mL) was detected in TB patients, wherein 88.2% and 81.1% sensitivities were obtained in pulmonary TB and extrapulmonary TB respectively, with 96-96.4% specificity. This assay may translate into a diagnostic kit.
一种实时免疫聚合酶链反应（immuno-PCR）检测法被用来检测分枝杆菌甘露磷酸肌醇（PIMs）。该检测法可检测到结核患者中 PIMs 的动态范围（0.9 pg/mL-10 ng/mL），其中肺结核和肺外结核的灵敏度分别为 88.2%和 81.1%，特异性为 96-96.4%。该检测法可能转化为一种诊断试剂盒。
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