Let-7c对乙型肝炎病毒相关肝病进程的影响。

Effects of Let-7c on the processing of hepatitis B virus associated liver diseases.

作者信息

Zhang Like, Jiang Xia, Wang Guiqi, Kanda Tatsuo, Yokosuka Osamu, Zhai Congjie, Zhang Lei, Liu Peng, Zhao Zengren, Li Zhongxin

机构信息

Department of General Surgery, Hebei Key Laboratory of Colorectal Cancer Precision Diagnosis and Treatment, The First Hospital of Hebei Medical University, No.89 Donggang Road, Shijiazhuang, 050031, Hebei, China.

Department of Gastroenterology and Nephrology, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, Japan.

出版信息

Infect Agent Cancer. 2022 Sep 3;17(1):46. doi: 10.1186/s13027-022-00458-8.

DOI:10.1186/s13027-022-00458-8

PMID:36057607

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9440497/

Abstract

BACKGROUND

The most common type of cancer of the digestive system is hepatocellular carcinoma. In China, many patients harbour HBV. The lin28B/Let-7c/MYC axis is associated with the occurrence of many cancers. Therefore, we aimed to illuminate the function of the lin28B/Let-7c/MYC axis in hepatocellular carcinoma. We aimed to evaluate the critical involvement of lin28B and Let-7c in the carcinogenesis of human hepatocellular carcinoma (B-HCC).

METHODS

Data from the GEO database were used to analyse differentially expressed genes and IRGs. A protein - protein interaction (PPI) network and Venn diagram were generated to analyse relationships. Real-time RT-PCR, Western blotting, and cell counting kit-8 assays were used to examine the association of lin28B, Let-7c, and MYC with cell proliferation.

RESULTS

A total of 2552 functionally annotated differentially expressed RNAs were analysed in HBV patients from the GSE135860 database. In addition, 46 let-7c target genes were screened in HBV patients, and the interactions were analysed through PPI network analysis. The results confirmed that Let-7c and its target genes play a key role in HBV-related diseases. Next, we discovered a gradual decrease in Let-7c expression during the progression from HBV-associated chronic hepatitis (B-CH) and HBV-associated liver cirrhosis (B-LC) to B-HCC. We found evidence for a negative association between lin28B expression and Let-7c expression. The expression of MYC was obviously upregulated when Let-7c was inhibited.

CONCLUSION

Our results highlight that Let-7c and lin28B participate in the carcinogenesis of HBV-associated diseases through the lin28B/Let-7c/MYC axis.

摘要

背景

消化系统最常见的癌症类型是肝细胞癌。在中国，许多患者感染乙肝病毒。lin28B/Let-7c/MYC轴与多种癌症的发生有关。因此，我们旨在阐明lin28B/Let-7c/MYC轴在肝细胞癌中的作用。我们旨在评估lin28B和Let-7c在人类肝细胞癌（B-HCC）致癌过程中的关键作用。

方法

使用来自GEO数据库的数据来分析差异表达基因和免疫相关基因。生成蛋白质-蛋白质相互作用（PPI）网络和维恩图以分析关系。使用实时逆转录PCR、蛋白质印迹和细胞计数试剂盒-8测定法来检测lin28B、Let-7c和MYC与细胞增殖的关联。

结果

在来自GSE135860数据库的乙肝患者中总共分析了2552个具有功能注释的差异表达RNA。此外，在乙肝患者中筛选出46个Let-7c靶基因，并通过PPI网络分析来分析相互作用。结果证实Let-7c及其靶基因在乙肝相关疾病中起关键作用。接下来，我们发现在从乙肝相关慢性肝炎（B-CH）和乙肝相关肝硬化（B-LC）进展到B-HCC的过程中Let-7c表达逐渐降低。我们发现lin28B表达与Let-7c表达之间存在负相关的证据。当Let-7c受到抑制时，MYC的表达明显上调。