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建立优化的小鼠原位膀胱癌模型。

Establishment of an optimized orthotopic bladder cancer model in mice.

机构信息

Department of Urology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, No. 85 Wujin Road, Hongkou District, Shanghai, 200080, China.

Department of Urology, Shanghai General Hospital Affiliated to Nanjing Medical University, Shanghai, 200080, China.

出版信息

BMC Urol. 2022 Sep 3;22(1):142. doi: 10.1186/s12894-022-01093-6.

DOI:10.1186/s12894-022-01093-6

PMID:36057655

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9441054/

Abstract

BACKGROUND

Bladder cancer (BC) is one of the most common malignancies of the genitourinary system. Animal models offer an important tool to explore tumour initiation, progression, and therapeutic mechanisms. Our aim is to construct an optimized orthotopic BC model which is predictable, reproducible, and convenient.

METHODS

The optimized orthotopic BC model was constructed in male C57BL/6 mice utilizing microsyringes to inoculate them with a murine BC cell line (MB49). Anesthetised mice were inoculated with an MB49 cell suspension (10 µL) at approximately 5 × 10/mL. The whole process of modelling was observed and monitored every 3 days for 21 days utilizing HE staining and transabdominal ultrasonography (TUS).

RESULTS

In this study, the model showed excellent success rates for tumour formation (96.67%) and metastatic rate (89.66%). Compared to the control group (sham operation), mice in the modelling group had serous cachexia, visible haematuresis and weight loss (all P < 0.05). The lungs, liver, ureter and kidneys were found to have tumour metastasis. Moreover, the average survival time (19.73 ± 1.69 d) of modelling mice was significantly shorter than that of the control mice (P < 0.05), which remained alive.

CONCLUSION

Our study established a method using microsyringes to inject murine BC cells into the bladder wall, creating a stable transplantable BC model in mice.

摘要

背景

膀胱癌（BC）是泌尿系统最常见的恶性肿瘤之一。动物模型为探索肿瘤的发生、进展和治疗机制提供了重要工具。我们的目的是构建一种可预测、可重复且方便的优化的原位膀胱癌模型。

方法

利用微量注射器将小鼠膀胱癌细胞系（MB49）接种到雄性 C57BL/6 小鼠的膀胱壁中，构建优化的原位膀胱癌模型。麻醉小鼠以约 5×10/ml 的浓度接种 MB49 细胞悬液（10 μL）。利用苏木精和伊红（HE）染色和经腹超声（TUS）监测，观察并监测建模的全过程，21 天内每 3 天监测一次。

结果

在这项研究中，该模型的肿瘤形成率（96.67%）和转移率（89.66%）非常高。与对照组（假手术）相比，建模组小鼠出现严重的恶病质、肉眼血尿和体重减轻（均 P<0.05）。肺部、肝脏、输尿管和肾脏均发现有肿瘤转移。此外，建模小鼠的平均存活时间（19.73±1.69 d）明显短于对照组仍存活的小鼠（P<0.05）。