DECOD (Ecosystem Dynamics and Sustainability), INRAE, Institut Agro, IFREMER, Rennes, France.
Management of Diadromous Fish in their Environment, OFB, INRAE, Institut Agro, Univ Pau & Pays Adour/E2S UPPA, Rennes, France.
Mol Biol Rep. 2022 Oct;49(10):10115-10119. doi: 10.1007/s11033-022-07800-8. Epub 2022 Sep 4.
The European River lamprey Lampetra fluviatilis and the brook lamprey L. planeri are two closely related species that are also considered as partially reproductively isolated ecotypes. At the larval stage, they cannot be distinguished morphologically or genetically by mitochondrial DNA. We aimed at developing a molecular tool to identify early life stages of L. fluviatilis and L. planeri.
We first identified Single Nucleotide Polymorphism (SNP) markers with distinct alleles between L. fluviatilis and L. planeri based on RADseq data from 186 individuals collected in France. Then, we developed a quantitative PCR protocol to genotype a species-specific SNP, which was tested on 270 samples including larvae and adults from both species and 17 sites from Western Europe. Results were consistent with morphological identification in all cases except for samples from the Rhône drainage and the Loch Lomond in Scotland.
This marker allows the identification of larval stages of L. fluviatilis, L. planeri as well as hybrids. The two cases where the marker was not diagnostic might be explained by an isolated and distinct L. planeri lineage in the Rhône drainage, and high levels of admixture among L. fluviatilis, L. planeri and the lake-parasitic form in Loch Lomond.
欧洲七鳃鳗 Lampetra fluviatilis 和溪红点鲑 L. planeri 是两种密切相关的物种，它们也被认为是部分生殖隔离的生态型。在幼虫阶段，它们在形态上或遗传上无法通过线粒体 DNA 区分。我们旨在开发一种分子工具来鉴定 L. fluviatilis 和 L. planeri 的早期生命阶段。
我们首先基于在法国收集的 186 个人的 RADseq 数据，确定了 L. fluviatilis 和 L. planeri 之间具有不同等位基因的单核苷酸多态性（SNP）标记。然后，我们开发了一种定量 PCR 协议来对一个物种特异性 SNP 进行基因分型，该协议在包括来自两个物种的幼虫和成虫以及来自西欧的 17 个地点的 270 个样本上进行了测试。除了来自罗纳河排水区和苏格兰洛蒙德湖的样本外，结果与形态鉴定一致。
该标记可用于鉴定 L. fluviatilis、L. planeri 及其杂种的幼虫阶段。标记未诊断的两种情况可能是由于罗纳河排水区的一个孤立和独特的 L. planeri 谱系，以及洛蒙德湖的 L. fluviatilis、L. planeri 和湖内寄生形式之间高水平的混合。
