Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Life Sci. 2022 Nov 1;308:120926. doi: 10.1016/j.lfs.2022.120926. Epub 2022 Sep 1.
Fatty liver hemorrhagic syndrome (FLHS) seriously threatens the layer industry due to it can cause a sudden decline in egg production and acute death, and dietary supplement with bioactive substance is considered an effective way to prevent the FLHS occurrence. Dehydroepiandrosterone (DHEA) is a popular dietary supplement and it possesses anti-oxidative and anti-inflammatory functions; however, the effect and underlying mechanism about DHEA in protecting against the occurrence and development of FLHS remain elucidated. The current results showed that DHEA relieved HELP-induced decrease of egg productivity and liver injury in laying hens. Meanwhile, DHEA markedly enhanced the antioxidant capacity and then alleviated oxidative stress via activation of nuclear factor (erythroid-derived 2)-like 2 (NRF-2) signal in laying hens fed with HELP diets. In addition, DHEA significantly alleviated HELP-stimulated systemic inflammatory response by suppressing the overproduction of hepatic pro-inflammatory factors in laying hens, and further found this beneficial effect was achieved by blocking the activation of NF-κB pathway. Furthermore, we found that DHEA promoted the AMP-activated protein kinase α (AMPKα) activation and increased the G-protein-coupled estrogen receptor (GPER) expression level in laying hens fed with HELP diets. In summary, our data demonstrated that DHEA attenuates oxidative stress and inflammation through the activation of GPER-AMPK signal axis in laying hens fed with HELP diets. These results might facilitate an understanding of the benefits and mechanism of DHEA on the development of FLHS, and provide sufficient data to support it as a dietary supplement to control the FLHS-related metabolic diseases in chickens.
脂肪肝出血综合征（FLHS）可导致产蛋率突然下降和急性死亡，严重威胁蛋鸡养殖业，而在饲料中添加具有生物活性的物质被认为是预防 FLHS 发生的有效方法。脱氢表雄酮（DHEA）是一种常用的膳食补充剂，具有抗氧化和抗炎作用；然而，DHEA 预防 FLHS 发生和发展的作用及其潜在机制尚不清楚。本研究结果表明，DHEA 缓解了 HELP 诱导的产蛋鸡产蛋性能下降和肝损伤。同时，DHEA 通过激活核因子（红系衍生 2）样 2（NRF-2）信号，显著增强了抗氧化能力，从而减轻了氧化应激，在喂食 HELP 日粮的母鸡中。此外，DHEA 通过抑制肝内促炎因子的过度产生，显著减轻了 HELP 刺激的全身炎症反应，进一步发现这种有益作用是通过阻断 NF-κB 途径的激活来实现的。此外，我们发现 DHEA 促进了 AMP 激活的蛋白激酶α（AMPKα）的激活，并增加了喂食 HELP 日粮的母鸡中 G 蛋白偶联雌激素受体（GPER）的表达水平。综上所述，我们的数据表明，DHEA 通过激活 GPER-AMPK 信号轴减轻了喂食 HELP 日粮的母鸡的氧化应激和炎症。这些结果可能有助于了解 DHEA 对 FLHS 发展的益处和机制，并为将其作为膳食补充剂来控制鸡中与 FLHS 相关的代谢疾病提供充分的数据支持。
