Department of Pediatrics, Affiliated Hospital of Jiangsu University, Zhenjiang, 212013, China; School of Medicine, Jiangsu University, Zhenjiang, 212013, China.
Department of Clinical Laboratory, Xuzhou Central Hospital, Xuzhou 221009, Jiangsu, China.
Virus Res. 2022 Nov;321:198912. doi: 10.1016/j.virusres.2022.198912. Epub 2022 Sep 2.
Acute respiratory tract infections are a major public health problem and the leading cause of morbidity in children younger than 5 years old. This study investigated the potential reasons of unexplained acute respiratory infections in children in Xuzhou and its environs during 2018-2019.We collected pharyngeal swab samples from 411 children under the age of five who presented with symptoms of unexplained acute respiratory infection and were negative for bacteria, mycoplasma, and influenza viruses. Using viral metagenomic techniques, viral nucleic acids were extracted, enriched, and sequenced from the samples. Results indicated that Picornaviridae, Parvoviridae, Paramyxoviridae, Coronaviridae, and Anelloviridae were the five virus families with the highest relative content of sequence reads. And we detected 35 HBoV-positive and 12 HEV-positive samples out of 411 samples by the polymerase chain reaction (PCR). Partial or nearly complete genome sequences of viruses belonging to the families Picornaviridae, Parvoviridae, and Anelloviridae were characterized, and phylogenetic trees were constructed based on the nucleic acid or amino acid sequences of the predicted viral open reading frames (ORFs), as well as genotyping of the viruses. In addition, we observed recombination events in the Saffold virus and Coxsackievirus A9 by analyzing the genetic characteristics of the viruses revealed in this study. This study provides vital information for the prevention and treatment of acute respiratory infections in children younger than five years old.
急性呼吸道感染是一个主要的公共卫生问题，也是 5 岁以下儿童发病率的主要原因。本研究调查了 2018-2019 年徐州及其周边地区儿童不明原因急性呼吸道感染的潜在原因。我们收集了 411 名 5 岁以下出现不明原因急性呼吸道感染症状且细菌、支原体和流感病毒检测均为阴性的患儿的咽拭子样本。采用病毒宏基因组技术，从样本中提取、富集和测序病毒核酸。结果表明，小核糖核酸病毒科、细小病毒科、副粘病毒科、冠状病毒科和圆环病毒科是序列读取相对含量最高的五个病毒科。通过聚合酶链反应（PCR），我们从 411 个样本中检测到 35 个 HBoV 阳性和 12 个 HEV 阳性样本。对属于小核糖核酸病毒科、细小病毒科和圆环病毒科的病毒的部分或几乎完整的基因组序列进行了特征描述，并根据预测的病毒开放阅读框（ORFs）的核酸或氨基酸序列以及病毒的基因分型构建了系统发育树。此外，通过分析本研究中揭示的病毒遗传特征，我们观察到 Saffold 病毒和柯萨奇病毒 A9 中的重组事件。本研究为 5 岁以下儿童急性呼吸道感染的预防和治疗提供了重要信息。
