Department of Aquatic Bioscience, Graduate School of Agriculture and Life Science, The University of Tokyo, Yayoi 1-1-1, Bunkyo, Tokyo 113-8657, Japan.
Department of Aquatic Bioscience, Graduate School of Agriculture and Life Science, The University of Tokyo, Yayoi 1-1-1, Bunkyo, Tokyo 113-8657, Japan; School of Biomedical Engineering, Faculty of Applied Science and Faculty of Medicine, The University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
Genomics. 2022 Sep;114(5):110477. doi: 10.1016/j.ygeno.2022.110477. Epub 2022 Sep 1.
Fish exhibit different muscle structures and growth characteristics compared with mammals. We used a spatial transcriptomics approach and examined myotomal muscle sections from zebrafish. Adult muscles were divided into eight regions according to spatial gene expression characteristics. Slow muscle was located in the wedge-shaped region near the lateral line and at the base of the dorsal fin, intermediate muscle was located in a ribbon-shaped region adjacent to slow muscle, and fast muscle was located in the deep region of the trunk, surrounded by intermediate muscle; the interior of fast muscle was further divided into 6 parts by their transcriptomic features. Combined analysis of adult and larval data revealed that adult muscles contain specific regions similar to larval muscles. These regions showed active myogenesis and a high expression of genes associated with muscle hyperplasia. This is the first study to apply spatial transcriptomics to fish myotomal muscle structure and growth.
鱼类的肌肉结构和生长特征与哺乳动物不同。我们采用空间转录组学方法，对斑马鱼的肌节肌肉切片进行了研究。根据空间基因表达特征，成年肌肉被分为八个区域。慢肌位于靠近侧线和背鳍基部的楔形区域，中间肌位于与慢肌相邻的带状区域，快肌位于躯干的深部，被中间肌包围；快肌的内部进一步根据转录组特征分为 6 部分。成年和幼体数据的综合分析表明，成年肌肉包含类似于幼体肌肉的特定区域。这些区域表现出活跃的肌生成和与肌肉增生相关的基因的高表达。这是首次将空间转录组学应用于鱼类肌节肌肉结构和生长的研究。
