Department of Vascular and Endovascular Surgery, General Hospital of People's Liberation Army, Beijing, Fuxing Road 28#, 100853, P.R. China.
Department of General Surgery, Affiliated Hospital of Chengde Medical University, Nanyingzi Street 36#, Chengde City, Hebei, 067000, P.R. China.
Exp Anim. 2023 Feb 21;72(1):38-46. doi: 10.1538/expanim.22-0020. Epub 2022 Sep 3.
Previous abdominal aortic aneurysm (AAA) animal modeling methodologies were either expensive or complicated. Here, we developed a novel AAA model which was simple to set up and generated minimal calcification. Twenty-four rats were divided randomly into four groups. Groups 1, 2 and 3 underwent surgery in which 15% hydrochloric acid (HCl) was applied periarterially to the abdominal aorta for 5 min, followed by sacrifice 1 week (group 1), 2 weeks (group 2), and 4 weeks (group 3) after surgery. The maximum aortic diameter (MAD) was measured at surgery and before animal sacrifice. Rats in group 4 were sham-treated. The MADs in group 1, 2 and 3 showed significant dilation compared with group 4, with a mean dilation rate of 33.8% in the first week after surgery. Histopathological examination revealed infiltration of macrophages into the adventitia, obvious apoptosis of smooth muscle cells, and rupture and collapse of the elastic fibers. Furthermore, no calcification was observed in the dilated aorta. The mRNA expression levels of inflammatory factors were at least two-fold higher in group 1 than in group 4, indicating significant inflammatory response in the progression of AAA information. In conclusion, periarterial application of 15% HCl is a convenient and reliable model to create an abdominal aortic aneurysm in rats, and the potential development mechanism may be related to the proinflammatory effects of HCl.
先前的腹主动脉瘤（AAA）动物模型建立方法要么昂贵，要么复杂。在这里，我们开发了一种新的 AAA 模型，该模型建立简单，且产生的钙化最小。24 只大鼠随机分为 4 组。第 1、2 和 3 组大鼠的腹主动脉周围接受 15%盐酸（HCl）处理 5 分钟，然后分别在手术后 1 周（第 1 组）、2 周（第 2 组）和 4 周（第 3 组）处死。在手术和动物处死前测量最大主动脉直径（MAD）。第 4 组大鼠为假手术处理组。第 1、2 和 3 组的 MAD 与第 4 组相比明显扩张，术后第 1 周扩张率平均为 33.8%。组织病理学检查显示巨噬细胞浸润到外膜，平滑肌细胞明显凋亡，弹性纤维破裂和塌陷。此外，扩张的主动脉中未见钙化。第 1 组大鼠的炎症因子 mRNA 表达水平至少是第 4 组的两倍，表明 AAA 进展过程中存在明显的炎症反应。总之，15% HCl 周围动脉内注射是一种方便可靠的大鼠腹主动脉瘤模型建立方法，其潜在的发展机制可能与 HCl 的促炎作用有关。
