State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu Province, 211198, People's Republic of China.
NMPA Key Laboratory for Bioequivalence Research of Generic Drug Evaluation, Shenzhen Institute for Drug Control, Shenzhen, Guangdong Province, 518057, People's Republic of China.
Int J Nanomedicine. 2022 Aug 26;17:3723-3733. doi: 10.2147/IJN.S377575. eCollection 2022.
Urinary tract infections (UTI) are one of the most serious human bacterial infections affecting millions of people every year. Therefore, simple and reliable identification of the urinary tract pathogenic bacteria within a few minutes would be of great significance for diagnosis and treatment of clinical patients with UTIs. In this study, the fluorescence sensor was reported to guide the detection of urinary tract bacterial infections rapidly.
The Ami-AuNPs-DNAs sensor was fabricated by the amino-modified Au nanoparticles (Ami-AuNPs) and six DNAs signal molecules, which bound to the urinary tract pathogenic bacteria and generated corresponding response signals. Further, based on the collected response signals, identification was performed by principal component analysis (PCA) and linear discriminant analysis (LDA). The Ami-AuNPs and Ami-AuNPs-DNAs were characterized by transmission electron microscopy, UV-vis absorption spectrum, Fourier transform infrared spectrum, dynamic light scattering and zeta potentials. Thereafter, the Ami-AuNPs-DNAs sensor was used to discriminate and identify five kinds of urinary tract pathogenic bacteria. Moreover, the quantitative analysis performance towards individual bacteria at different concentrations were also evaluated.
The Ami-AuNPs-DNAs sensor were synthesized successfully in terms of spherical, well-dispersed and uniform in size, which could well discriminate five main urinary tract pathogenic bacteria with unique fingerprint-like patterns and was sufficiently sensitive to determine individual bacteria with a detection limit to 1×10 cfu/mL. Furthermore, the sensor had also been successfully applied to identify bacteria in urine samples collected from clinical UTIs.
The developed fluorescence sensor could be applied to rapid and accurate discrimination of urinary tract pathogenic bacteria and holds great promise for the diagnosis of the disease caused by bacterial infection.
尿路感染（UTI）是每年影响数百万人的最严重的人类细菌感染之一。因此，能够在几分钟内快速可靠地鉴定尿路感染的尿路病原菌，对于临床尿路感染患者的诊断和治疗具有重要意义。在本研究中，报告了荧光传感器可用于快速指导尿路细菌感染的检测。
通过氨基修饰的金纳米粒子（Ami-AuNPs）和 6 个 DNA 信号分子构建了 Ami-AuNPs-DNAs 传感器，该传感器与尿路病原菌结合并产生相应的响应信号。进一步，基于收集的响应信号，通过主成分分析（PCA）和线性判别分析（LDA）进行鉴定。通过透射电子显微镜、紫外-可见吸收光谱、傅里叶变换红外光谱、动态光散射和 zeta 电位对 Ami-AuNPs 和 Ami-AuNPs-DNAs 进行了表征。此后，使用 Ami-AuNPs-DNAs 传感器来区分和鉴定五种尿路病原菌。此外，还评估了该传感器对不同浓度的单个细菌的定量分析性能。
成功合成了 Ami-AuNPs-DNAs 传感器，该传感器呈球形，分散良好且尺寸均匀，能够很好地区分五种主要的尿路病原菌，具有独特的指纹样模式，并且足够灵敏，能够以 1×10 cfu/mL 的检测限确定单个细菌。此外，该传感器还成功应用于鉴定来自临床 UTI 尿液样本中的细菌。
所开发的荧光传感器可用于快速准确地鉴别尿路病原菌，有望用于由细菌感染引起的疾病的诊断。
