Dobrowolski Adam, Nawijn Willem, Mirończuk Aleksandra M
Department of Biotechnology and Food Microbiology, Faculty of Biotechnology and Food Science, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
Front Bioeng Biotechnol. 2022 Aug 17;10:944228. doi: 10.3389/fbioe.2022.944228. eCollection 2022.
Biomass of the brown algae and is a promising, renewable feedstock because of the high growth rate, accessibility and content of glucose and mannitol. Saccharification of seaweeds is a simple process due to the lack of lignocellulose in the cell wall. The high content of glucose and mannitol makes these seaweeds an attractive feedstock for lipid production in the yeast This study demonstrated that hydrolysates of brown algae biomass can be applied as a substrate for synthesis of yeast biomass and lipids without any supplementation. To increase the lipid titer in yeast biomass, we employed an engineered strain of overexpressing DGA1/DGA2. In consequence, the C/N ratio has a lower impact on lipid synthesis. Moreover, the applied substrates allowed for high synthesis of unsaturated fatty acids (UFA); the level exceeded 90% in the fatty acid pool. Oleic (C18:1) and linoleic acids (C18:2) achieved the highest content. The study showed that is able to grow on the seaweed hydrolysate and produces a high content of UFA in the biomass.
褐藻的生物质是一种很有前景的可再生原料，因为其生长速度快、易于获取，且葡萄糖和甘露醇含量高。由于细胞壁中缺乏木质纤维素，海藻的糖化过程很简单。葡萄糖和甘露醇的高含量使这些海藻成为酵母中脂质生产的有吸引力的原料。本研究表明，褐藻生物质水解产物无需任何补充即可用作合成酵母生物质和脂质的底物。为了提高酵母生物质中的脂质滴度，我们使用了过表达DGA1/DGA2的工程菌株。因此，碳氮比对脂质合成的影响较小。此外，所应用的底物允许高度合成不饱和脂肪酸（UFA）；脂肪酸池中该水平超过90%。油酸（C18:1）和亚油酸（C18:2）含量最高。该研究表明，[此处原文有缺失内容]能够在海藻水解产物上生长，并在生物质中产生高含量的UFA。
