Katano M, Yamamoto H, Torisu M
Gan To Kagaku Ryoho. 1987 Jul;14(7):2321-6.
We found that PSK has ambidextrous effects on cell motility. PSK enhances macrophage motility and inhibits tumor cell motility, when the capillary tube method was used in vitro. Macrophage motility was enhanced with increasing concentration of PSK. PSK inhibited tumor cell motility, i.e. Ehrlich tumor cells, EL-4 lymphoma cells and human leukemic cells, in dose dependent fashion. Macrophages and tumor cells were incubated with medium containing PSK for varying times at 37 degrees C and then washed with medium to eliminate PSK thoroughly. The motility of macrophages and tumor cells was enhanced and inhibited, respectively, with increasing pre-incubation time. When PSK-treated tumor cells were injected into the abdominal wall of C57BL/6 mice, PSK-treated tumor cells were less invasive than non-treated ones in mice. These phenomena are concern neither with the change of cellular viability nor that of cell proliferation.
我们发现PSK对细胞运动具有双向作用。当在体外使用毛细管法时，PSK增强巨噬细胞运动并抑制肿瘤细胞运动。随着PSK浓度增加，巨噬细胞运动增强。PSK以剂量依赖方式抑制肿瘤细胞运动，即艾氏瘤细胞、EL-4淋巴瘤细胞和人白血病细胞。巨噬细胞和肿瘤细胞在含有PSK的培养基中于37℃孵育不同时间，然后用培养基洗涤以彻底去除PSK。随着预孵育时间增加，巨噬细胞和肿瘤细胞的运动分别增强和受到抑制。当将经PSK处理的肿瘤细胞注射到C57BL/6小鼠的腹壁中时，经PSK处理的肿瘤细胞在小鼠中的侵袭性低于未处理的肿瘤细胞。这些现象既与细胞活力的变化无关，也与细胞增殖的变化无关。
