Wang Kexin, Yuan Yuhao, Luo Xinyu, Shen Zhaoyang, Huang Yinghui, Zhou Haolu, Gao Xiaoli
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China.
Front Plant Sci. 2022 Aug 18;13:961447. doi: 10.3389/fpls.2022.961447. eCollection 2022.
Selenium (Se) biofortification is an important strategy for reducing hidden hunger by increasing the nutritional quality of crops. However, there is limited metabolomic information on the nutritional quality of Se-enriched mung beans. In this study, physiological assays and LC-MS/MS based widely targeted metabolomics approach was employed to reveal the Se biofortification potential of mung bean by evaluating the effect of Se on mung bean nutraceutical compounds and their qualitative parameters. Physiological data showed that foliar application of 30 g ha Se at key growth stages significantly increased the content of Se, protein, fat, total phenols, and total flavonoids content in two mung bean varieties. Widely targeted metabolomics identified 1,080 metabolites, among which L-Alanyl-L-leucine, 9,10-Dihydroxy-12,13-epoxyoctadecanoic acid, and 1-caffeoylquinic acid could serve as biomarkers for identifying highly nutritious mung bean varieties. Pathway enrichment analysis revealed that the metabolic pathways of different metabolites were different in the Se-enriched mung bean. Specifically, P1 was mainly enriched in the linoleic acid metabolic pathway, while P2 was mainly enriched in the phosphonate and phosphinate metabolic pathways. Overall, these results revealed the specific Se enrichment mechanism of different mung bean varieties. This study provides new insights into the comprehensive improvement of the nutritional quality of mung beans.
硒（Se）生物强化是通过提高作物营养品质来减少隐性饥饿的一项重要策略。然而，关于富硒绿豆营养品质的代谢组学信息有限。在本研究中，采用生理测定和基于液相色谱-串联质谱（LC-MS/MS）的广泛靶向代谢组学方法，通过评估硒对绿豆营养成分及其定性参数的影响，揭示绿豆的硒生物强化潜力。生理数据表明，在关键生长阶段叶面喷施30 g·ha硒显著提高了两个绿豆品种中硒、蛋白质、脂肪、总酚和总黄酮的含量。广泛靶向代谢组学鉴定出1080种代谢物，其中L-丙氨酰-L-亮氨酸、9,10-二羟基-12,13-环氧十八烷酸和1-咖啡酰奎尼酸可作为鉴定高营养绿豆品种的生物标志物。通路富集分析表明，富硒绿豆中不同代谢物的代谢通路存在差异。具体而言，P1主要富集于亚油酸代谢通路，而P2主要富集于膦酸酯和次膦酸酯代谢通路。总体而言，这些结果揭示了不同绿豆品种特定的硒富集机制。本研究为全面改善绿豆营养品质提供了新的见解。
