• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

雌球花发育过程中的形态组织学、内源激素动态及转录组分析

Morpho-histology, endogenous hormone dynamics, and transcriptome profiling in during female cone development.

作者信息

Wang Enbo, Lu Wenju, Liang Haiying, Zhang Xumeng, Huo Shaojie, Song Xiqiang, Wang Jian, Zhao Ying

机构信息

Key Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants, Key Laboratory of Germplasm Resources Biology of Tropical Special Ornamental Plants of Hainan Province, College of Forestry, Hainan University, Haikou, China.

Department of Genetics and Biochemistry, Clemson University, Clemson, SC, United States.

出版信息

Front Plant Sci. 2022 Aug 17;13:954788. doi: 10.3389/fpls.2022.954788. eCollection 2022.

DOI:10.3389/fpls.2022.954788
PMID:36061797
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9428629/
Abstract

de Laubenfels is a perennial dioeciously gymnosperm species dominant in tropical montane rain forests. Due to deforestation, natural disasters, long infancy, and poor natural regeneration ability, the population of this species has been significantly reduced and listed as an endangered protected plant. To better understand the female cone development in , we examined the morphological and anatomical changes, analyzed the endogenous hormone dynamics, and profiled gene expression. The female reproductive structures were first observed in January. The morpho-histological observations suggest that the development of the megaspore can be largely divided into six stages: early flower bud differentiation, bract primordium differentiation, ovule primordium differentiation, dormancy, ovule maturity, and seed maturity. The levels of gibberellins (GA), auxin (IAA), abscisic acid (ABA), and cytokinin (CTK) fluctuate during the process of female cone development. The female cones of need to maintain a low level of GA-IAA-ABA steady state to promote seed germination. The first transcriptome database for female was generated, revealing 310,621 unigenes. Differential expression analyses revealed several floral (, , and ) and hormone biosynthesis and signal transduction (, , , , , etc.) genes that could be critical for female cone development. Our study provides new insights into the cone development in and the foundation for female cone induction with hormones.

摘要

德劳本费尔斯松是一种多年生雌雄异株的裸子植物，在热带山地雨林中占主导地位。由于森林砍伐、自然灾害、生长周期长以及自然更新能力差，该物种的种群数量已大幅减少，并被列为濒危保护植物。为了更好地了解德劳本费尔斯松雌球果的发育过程，我们研究了其形态和解剖结构的变化，分析了内源激素动态，并对基因表达进行了分析。雌球果最早在1月被观察到。形态组织学观察表明，大孢子的发育大致可分为六个阶段：花芽早期分化、苞片原基分化、胚珠原基分化、休眠、胚珠成熟和种子成熟。在雌球果发育过程中，赤霉素（GA）、生长素（IAA）、脱落酸（ABA）和细胞分裂素（CTK）的水平会发生波动。德劳本费尔斯松的雌球果需要维持低水平的GA-IAA-ABA稳态以促进种子萌发。我们构建了首个德劳本费尔斯松雌球果转录组数据库，共鉴定出310,621个单基因。差异表达分析揭示了几个可能对雌球果发育至关重要的花发育（如AP1、FUL和SEP等）以及激素生物合成和信号转导（如GA20ox、GA3ox、PIN、ABF、ARF等）相关基因。我们的研究为德劳本费尔斯松球果发育提供了新的见解，并为利用激素诱导雌球果奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/997b0ed9c425/fpls-13-954788-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/e67c03113af9/fpls-13-954788-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/02bb86713c12/fpls-13-954788-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/5df4442fce19/fpls-13-954788-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/b8a162073cf3/fpls-13-954788-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/6efb2d94d995/fpls-13-954788-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/14992edf8a72/fpls-13-954788-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/ddbce77a8fe3/fpls-13-954788-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/71d8c84c85a8/fpls-13-954788-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/f1f29c989628/fpls-13-954788-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/997b0ed9c425/fpls-13-954788-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/e67c03113af9/fpls-13-954788-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/02bb86713c12/fpls-13-954788-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/5df4442fce19/fpls-13-954788-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/b8a162073cf3/fpls-13-954788-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/6efb2d94d995/fpls-13-954788-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/14992edf8a72/fpls-13-954788-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/ddbce77a8fe3/fpls-13-954788-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/71d8c84c85a8/fpls-13-954788-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/f1f29c989628/fpls-13-954788-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c377/9428629/997b0ed9c425/fpls-13-954788-g010.jpg

相似文献

1
Morpho-histology, endogenous hormone dynamics, and transcriptome profiling in during female cone development.雌球花发育过程中的形态组织学、内源激素动态及转录组分析
Front Plant Sci. 2022 Aug 17;13:954788. doi: 10.3389/fpls.2022.954788. eCollection 2022.
2
Transcriptome Analysis Reveals Endogenous Hormone Changes during Spike Development in .转录组分析揭示了 在穗发育过程中内源激素的变化 。
Int J Mol Sci. 2022 Sep 9;23(18):10461. doi: 10.3390/ijms231810461.
3
Identification of genes involved in metabolism and signalling of abscisic acid and gibberellins during Epimedium pseudowushanense B.L.Guo seed morphophysiological dormancy.鉴定淫羊藿种子形态生理休眠过程中脱落酸和赤霉素代谢和信号转导相关基因。
Plant Cell Rep. 2018 Jul;37(7):1061-1075. doi: 10.1007/s00299-018-2291-8. Epub 2018 May 23.
4
Comparative transcriptome analysis revealing the potential mechanism of seed germination stimulated by exogenous gibberellin in Fraxinus hupehensis.比较转录组分析揭示外源赤霉素刺激翅果油树种子萌发的潜在机制。
BMC Plant Biol. 2019 May 15;19(1):199. doi: 10.1186/s12870-019-1801-3.
5
Transcriptome and metabolome analyses reveal novel insights into the seed germination of Michelia chapensis, an endangered species in China.转录组和代谢组分析揭示了中国濒危物种长柄合果木种子萌发的新见解。
Plant Sci. 2023 Mar;328:111568. doi: 10.1016/j.plantsci.2022.111568. Epub 2022 Dec 15.
6
Phytohormone profiles and related gene expressions after endodormancy release in developing Pinus tabuliformis male strobili.发育中的华山松雄球果解除内休眠后植物激素的变化及其相关基因的表达。
Plant Sci. 2022 Mar;316:111167. doi: 10.1016/j.plantsci.2021.111167. Epub 2021 Dec 27.
7
Transcriptome and Metabolite Conjoint Analysis Reveals the Seed Dormancy Release Process in Callery Pear.转录组和代谢物联合分析揭示了西洋梨种子休眠释放过程。
Int J Mol Sci. 2022 Feb 16;23(4):2186. doi: 10.3390/ijms23042186.
8
Transcriptome Analysis Reveals the Role of GA in Regulating the Asynchronism of Floral Bud Differentiation and Development in Heterodichogamous (Batal.) Iljinskaja.转录组分析揭示 GA 在调控异型花柱 (Batal.) Iljinskaja 雌雄花芽分化和发育不同步中的作用。
Int J Mol Sci. 2022 Jun 17;23(12):6763. doi: 10.3390/ijms23126763.
9
Melatonin promotes seed germination under salt stress by regulating ABA and GA in cotton (Gossypium hirsutum L.).褪黑素通过调节棉花（Gossypium hirsutum L.）中的 ABA 和 GA 促进种子在盐胁迫下的萌发。
Plant Physiol Biochem. 2021 May;162:506-516. doi: 10.1016/j.plaphy.2021.03.029. Epub 2021 Mar 19.
10
Comprehensive Transcriptome Analysis of Phytohormone Biosynthesis and Signaling Genes in the Flowers of Chinese Chinquapin (Castanea henryi).锥栗（Castanea henryi）花中植物激素生物合成及信号转导基因的综合转录组分析
J Agric Food Chem. 2017 Nov 29;65(47):10332-10349. doi: 10.1021/acs.jafc.7b03755. Epub 2017 Nov 15.

引用本文的文献

1
Endogenous Hormone Regulation During Key Developmental Stages of Siebold & Zucc. Ovules.玉簪属胚珠关键发育阶段的内源激素调控
Plants (Basel). 2025 Feb 20;14(5):637. doi: 10.3390/plants14050637.
2
Comparative transcriptome analysis of dioecious floral development in Trachycarpus fortunei using Illumina and PacBio SMRT sequencing.利用 Illumina 和 PacBio SMRT 测序技术对掌叶木雌雄异株花发育的比较转录组分析。
BMC Plant Biol. 2023 Nov 3;23(1):536. doi: 10.1186/s12870-023-04551-x.

本文引用的文献

1
The Cycas genome and the early evolution of seed plants.苏铁基因组与种子植物的早期演化
Nat Plants. 2022 Apr;8(4):389-401. doi: 10.1038/s41477-022-01129-7. Epub 2022 Apr 18.
2
The Chinese pine genome and methylome unveil key features of conifer evolution.中国松基因组和甲基组揭示了针叶树进化的关键特征。
Cell. 2022 Jan 6;185(1):204-217.e14. doi: 10.1016/j.cell.2021.12.006. Epub 2021 Dec 28.
3
Characterization and Application of EST-SSR Markers Developed From the Transcriptome of (Taxaceae), a Relict Vulnerable Conifer.
从珍稀濒危针叶树红豆杉（Taxaceae）转录组开发的EST-SSR标记的特征与应用
Front Genet. 2019 Oct 18;10:1014. doi: 10.3389/fgene.2019.01014. eCollection 2019.
4
Transcriptome Analysis of Floral Buds Reveals the Role of Hormones and Transcription Factors in the Differentiation Process.花芽的转录组分析揭示了激素和转录因子在分化过程中的作用。
G3 (Bethesda). 2018 Mar 28;8(4):1103-1114. doi: 10.1534/g3.117.300481.
5
Changes in hormone flux and signaling in white spruce (Picea glauca) seeds during the transition from dormancy to germination in response to temperature cues.白云杉（Picea glauca）种子在从休眠到萌发的转变过程中，响应温度信号时激素通量和信号传导的变化。
BMC Plant Biol. 2015 Dec 18;15:292. doi: 10.1186/s12870-015-0638-7.
6
The link between flowering time and stress tolerance.开花时间与抗逆性之间的联系。
J Exp Bot. 2016 Jan;67(1):47-60. doi: 10.1093/jxb/erv441. Epub 2015 Oct 1.
7
A transcriptomics investigation into pine reproductive organ development.一项关于松树生殖器官发育的转录组学研究。
New Phytol. 2016 Feb;209(3):1278-89. doi: 10.1111/nph.13680. Epub 2015 Sep 25.
8
An RNA sequencing transcriptome analysis of the high-temperature stressed tall fescue reveals novel insights into plant thermotolerance.对高温胁迫下的高羊茅进行RNA测序转录组分析，揭示了植物耐热性的新见解。
BMC Genomics. 2014 Dec 19;15(1):1147. doi: 10.1186/1471-2164-15-1147.
9
Characterization of TM8, a MADS-box gene expressed in tomato flowers.TM8的特性分析，TM8是一个在番茄花中表达的MADS盒基因。
BMC Plant Biol. 2014 Nov 30;14:319. doi: 10.1186/s12870-014-0319-y.
10
Antimicrobial activity, toxicity and selectivity index of two biflavonoids and a flavone isolated from Podocarpus henkelii (Podocarpaceae) leaves.从罗汉松（罗汉松科）叶中分离得到的两种双黄酮和一种黄酮的抗菌活性、毒性和选择性指数。
BMC Complement Altern Med. 2014 Oct 8;14:383. doi: 10.1186/1472-6882-14-383.