Department of Physics and Astronomy, Washburn University, 1700 SW College Ave, Topeka, KS 66621, USA.
Department of Biology, Washburn University, 1700 SW College Ave, Topeka, KS 66621, USA.
Appl Opt. 2022 Jun 10;61(17):5282-5288. doi: 10.1364/ao.460443. Epub 2022 Jun 9.
In order to image live cells for prolonged periods of time, an Arduino-based, low-cost imaging incubator was constructed. The imaging incubator keeps cells viable by controlling for temperature and in order to maintain physiological conditions for cells during imaging. All devices and parts employed in the build were typical maker-type components in order to minimize the cost of the imaging incubator. The imaging incubator allows for real-time imaging of live cells exposed to any desired perturbation or stimulus. As a proof of the system's functionality, cells are imaged over 24 hours while remaining viable in the imaging incubator.
为了长时间对活细胞进行成像，我们构建了一个基于 Arduino 的低成本成像培养箱。该成像培养箱通过控制温度和湿度来保持细胞的活力，从而在成像过程中维持细胞的生理条件。构建中使用的所有设备和部件都是典型的制造商类型的组件，以最大限度地降低成像培养箱的成本。该成像培养箱允许对暴露于任何所需扰动或刺激的活细胞进行实时成像。作为系统功能的证明，细胞在成像培养箱中保持活力的情况下，可进行 24 小时的成像。
