用光镊探测染色质与染色质相关复合物的相互作用。
Probing the Interaction Between Chromatin and Chromatin-Associated Complexes with Optical Tweezers.
机构信息
Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University, New York, NY, USA.
Tri-Institutional PhD Program in Chemical Biology, New York, NY, USA.
出版信息
Methods Mol Biol. 2022;2478:313-327. doi: 10.1007/978-1-0716-2229-2_11.
Single-molecule force spectroscopy is a powerful tool to analyze the architecture and interaction of large macromolecular assemblies that are refractory to high-resolution structural interrogations. Here, we describe an optical tweezers-based platform for extracting the mechanical fingerprints of individual nucleosome arrays bound with chromatin-associated complexes, such as the Polycomb repressive complex 2 (PRC2). This platform comprehensively characterizes the diverse binding modes of PRC2 on chromatin, measures their mechanical strengths, and is broadly applicable to the studies of other epigenetic machineries.
单分子力谱是一种强大的工具，可用于分析对高分辨率结构探测具有抗性的大型大分子组装体的结构和相互作用。在这里，我们描述了一种基于光学镊子的平台，用于提取与染色质相关复合物（如多梳抑制复合物 2（PRC2））结合的单个核小体阵列的机械指纹。该平台全面表征了 PRC2 在染色质上的多种结合模式，测量了它们的机械强度，并且广泛适用于其他表观遗传机制的研究。
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