Colvin R B, Preffer F I
Arch Pathol Lab Med. 1987 Jul;111(7):628-32.
Automated flow cytometry provides an efficient, sensitive, objective, and quantitative means to analyze cells and their components in suspension. Two major diagnostic applications are the identification of cells in the blood by monoclonal antibodies to surface molecules and the characterization of tumor cell ploidy by nuclear DNA analysis. New technologies permit analysis of small quantities of cells (as in fine-needle aspirates), multiple simultaneous markers, oncogene products, and paraffin-embedded tissue. The technique has become the standard for cells normally in suspension (blood, other fluids). For cells readily obtained in suspension (fine-needle aspirates, nuclei), flow cytometry offers a practical alternative to computer-assisted microscopy.
自动流式细胞术提供了一种高效、灵敏、客观且定量的方法，用于分析悬浮液中的细胞及其成分。两项主要的诊断应用是通过针对表面分子的单克隆抗体识别血液中的细胞，以及通过核DNA分析表征肿瘤细胞的倍性。新技术允许分析少量细胞（如细针穿刺抽吸物）、多个同时标记物、癌基因产物和石蜡包埋组织。该技术已成为悬浮液中正常细胞（血液、其他体液）分析的标准方法。对于易于在悬浮液中获得的细胞（细针穿刺抽吸物、细胞核），流式细胞术为计算机辅助显微镜检查提供了一种实用的替代方法。
