Embrapa Uva e Vinho, Bento Gonçalves, Brazil.
Departamento de Ciência e Tecnologia Agroindustrial, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, Pelotas, Brazil.
J Sci Food Agric. 2023 Jan 30;103(2):576-589. doi: 10.1002/jsfa.12169. Epub 2022 Sep 5.
The year-round availability of apples (Malus × domestica Borkh.) depends on post-harvest technologies, which are essential for the retention of fruit sensory and chemical properties by delaying senescence. The effectiveness of strategies for preserving the quality of apples depends on complex interactions between the storage environment and endogenous biological factors. In the current work, we integrated instrumental, sensory, and transcriptional data to determine the role of conservation technologies cold storage, controlled atmosphere, and 1-methylcyclopropene-mediated ethylene blockage on the long-term conservation of apples.
The results demonstrated that inhibition of the consumer's perception of the apples' ethylene content is essential for long-term cold storage, and such quality conservation can be achieved by reducing oxygen pressure. Overall appreciation of apples after storage was determined mainly by their texture, with crispness and juiciness contributing favorably, and mealiness contributing negatively. Reduced oxygen pressure and inhibition of ethylene perception exerted distinct effects on the transcription of candidate genes associated with ripening in apple. Hexose and cell-wall carbohydrate metabolism genes exhibit distinct expression patterns under storage.
Inhibition of ethylene perception and reduction of relative oxygen pressure under cold storage both promote similar conservation of apple sensory traits under long-term cold storage. Texture was the main contributor to global appreciation of apples subjected to long-term storage. The conditions that were investigated were able to delay, but not fully prevent, senescence, as evidenced by physicochemical and gene expression analyses. The expression of gene-encoding enzymes involved in hexose metabolism was mainly developmentally regulated, whereas storage conditions exerted a stronger effect on the expression of genes associated with cell-wall metabolism. © 2022 Society of Chemical Industry.
苹果（Malus × domestica Borkh.）的全年供应依赖于采后技术，这些技术对于通过延缓衰老来保持果实的感官和化学特性至关重要。保持苹果品质的策略的有效性取决于储存环境和内源性生物因素之间的复杂相互作用。在当前的工作中，我们整合了仪器、感官和转录数据，以确定冷藏、控制气氛和 1-甲基环丙烯介导的乙烯阻断等保鲜技术在苹果的长期保鲜中的作用。
结果表明，抑制消费者对苹果乙烯含量的感知对于长期冷藏至关重要，通过降低氧气压力可以实现这种质量保存。贮藏后苹果的整体评价主要取决于其质地，脆度和多汁性有利，粉质感不利。降低氧气压力和抑制乙烯感知对与苹果成熟相关的候选基因的转录有明显的影响。己糖和细胞壁碳水化合物代谢基因在贮藏过程中表现出不同的表达模式。
冷藏过程中抑制乙烯感知和降低相对氧气压力均能促进苹果感官特性在长期冷藏中的相似保存。在长期贮藏过程中，质地是整体评价苹果的主要因素。研究的条件能够延缓衰老，但不能完全阻止衰老，这可以通过理化分析和基因表达分析来证明。参与己糖代谢的基因编码酶的表达主要受发育调控，而贮藏条件对与细胞壁代谢相关的基因表达有更强的影响。© 2022 化学工业协会。
