Department of Animal Science, Kashmar Branch, Islamic Azad University, Kashmar, Iran.
Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Vet Med Sci. 2022 Nov;8(6):2494-2503. doi: 10.1002/vms3.910. Epub 2022 Sep 5.
There have been some reports regarding supplementation of saffron petal extract on performance and egg quality in laying hens. However, the effect of saffron petal extract fed diet at different amounts on antioxidant status of laying hens and the impact of the resulting egg yolk on growth/inhibitory activity of cancer cells has not been fully studied.
The effect of dried saffron petal extract on the laying performance, egg qualitative traits, antioxidant status, and its inhibitory effect on cancer cells was studied.
A total of 200 39-week-old Hy-line W36 Leghorn laying hens were selected based on a completely randomised design in four treatments with five replications (10 hens per replication). The four treatment diets consisted of a basal diet with no supplement (control), and three diet supplement groups containing 40, 60 and 80 ppm of saffron petal extract.
Adding 80 ppm of saffron petal extract to layer diets increased egg production (p < 0.05). Malondialdehyde,1,1-diphenyl-2-picrylhydrazyl value and Superoxide dismutase significantly improved by saffron petal dietary supplementation. The yolk weight and colour, Haugh unit and shell weight and thickness were also influenced (p < 0.05) with highest values achieved in the 60 ppm saffron extract supplemented diet. Results demonstrated a significant effect of saffron extract inclusion in the diet on the growth of Michigan Cancer Foundation-7 and Prostate Cancer Cell in a positive dose-dependent manner (p < 0.05) and the most intense inhibitory effect on cancer cells was observed with 60-80 ppm extract.
Saffron petal extract can be used to potentially modulate the antioxidant status of laying hens and the inhibitory effect on cancer cells, best achieved with 60-80 ppm extract.
已有一些关于藏红花花瓣提取物对产蛋鸡生产性能和蛋品质影响的报道。然而，不同添加量的藏红花花瓣提取物对产蛋鸡抗氧化状态的影响以及由此产生的蛋黄对癌细胞生长/抑制活性的影响尚未得到充分研究。
研究干藏红花花瓣提取物对产蛋性能、蛋品质特性、抗氧化状态的影响及其对癌细胞的抑制作用。
采用完全随机设计，选择 200 只 39 周龄海兰 W36 褐壳蛋鸡，分为 4 个处理，每个处理 5 个重复，每个重复 10 只鸡。4 种处理日粮分别为基础日粮（对照组）和 3 种添加 40、60 和 80 ppm 藏红花花瓣提取物的日粮。
在产蛋鸡日粮中添加 80 ppm 的藏红花花瓣提取物可提高产蛋率（p < 0.05）。藏红花花瓣日粮添加可显著提高丙二醛、1,1-二苯基-2-苦基肼值和超氧化物歧化酶水平。蛋黄重量和颜色、哈夫单位、蛋壳重量和厚度也受到影响（p < 0.05），其中 60 ppm 藏红花提取物添加日粮的效果最佳。结果表明，藏红花提取物在日粮中的添加对密歇根癌症基金会-7 和前列腺癌细胞的生长具有显著的正向剂量依赖性影响（p < 0.05），且 60-80 ppm 提取物对癌细胞的抑制作用最强。
藏红花花瓣提取物可用于调节产蛋鸡的抗氧化状态和对癌细胞的抑制作用，60-80 ppm 提取物效果最佳。
