Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food of Ministry and Rural Affairs, College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
College of Food Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, China.
Food Chem. 2023 Jan 30;400:134028. doi: 10.1016/j.foodchem.2022.134028. Epub 2022 Aug 27.
This study aimed to develop and evaluate a method for simultaneously analyzing malondialdehyde (MDA) and two typical toxic α,β-unsaturated aldehydes, 4-hydroxy-2-hexenal (HHE), and 4-hydroxy-2-nonenal (HNE), using ultra-high-performance liquid chromatography with triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS). The method possessed widely linear range (10-1000 ng/mL). The limit of detection (LOD) and limit of quantitation (LOQ) of MDA, HHE, and HNE were 2.0 and 5.0 ng/g, 2.0 and 5.0 ng/g, and 0.1 and 0.3 ng/g, respectively; the recovery rates all fall into 95.56-104.22 %. The method was sufficiently precise (<5%), and not affected by the analysis matrix. Application to 17 food products revealed total MDA, HHE, and HNE contents were 0.11-3.56, 0.05-3.32, and 0.09-3.70 μg/g, respectively. It will be useful in future research on the influence of food composition and main substrate structure on the generation and distribution of these three aldehydes and the implementation of corresponding control methods.
本研究旨在开发并评估一种使用超高效液相色谱-三重四极杆质谱联用仪（UHPLC-QqQ-MS/MS）同时分析丙二醛（MDA）和两种典型的有毒α，β-不饱和醛，4-羟基-2-己烯醛（HHE）和 4-羟基-2-壬烯醛（HNE）的方法。该方法具有广泛的线性范围（10-1000ng/mL）。MDA、HHE 和 HNE 的检测限（LOD）和定量限（LOQ）分别为 2.0 和 5.0ng/g、2.0 和 5.0ng/g 以及 0.1 和 0.3ng/g；回收率均在 95.56-104.22%之间。该方法具有足够的精密度（<5%），不受分析基质的影响。应用于 17 种食品，发现总 MDA、HHE 和 HNE 的含量分别为 0.11-3.56μg/g、0.05-3.32μg/g 和 0.09-3.70μg/g。该方法将有助于未来研究食品成分和主要基质结构对这三种醛生成和分布的影响，并实施相应的控制方法。
