Department of Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
Department of Orthopaedic, Huizhou First Hospital, Guangdong Medical University, Huizhou, 516003 Guangdong, China.
J Immunol Res. 2022 Aug 27;2022:6568278. doi: 10.1155/2022/6568278. eCollection 2022.
The osteosarcoma (OS) microenvironment is composed of tumor cells, immune cells, and stromal tissue and is emerging as a pivotal player in OS development and progression. Thus, microenvironment-targeted strategies are urgently needed to improve OS treatment outcomes. Using principal component analysis (PCA), we systematically examined the tumor microenvironment (TME) and immune cell infiltration of 88 OS cases and constructed a TME scoring system based on the TMEscore high and TMEscore low phenotypes. Our analysis revealed that TMEscore high correlates with longer survival in OS patients, elevated immune cell infiltration, increased immune checkpoints, and increased sensitivity to chemotherapy. TMEscore low strongly correlated with immune exclusion. These observations were externally validated using a GEO dataset (GSE21257) from 53 OS patients. Our laboratory data also proved our findings. This finding enhances our understanding of the immunological landscape in OS and may uncover novel targeted therapeutic strategies.
骨肉瘤（OS）的微环境由肿瘤细胞、免疫细胞和基质组织组成，它正成为 OS 发展和进展的关键因素。因此，迫切需要针对微环境的策略来改善 OS 的治疗效果。我们使用主成分分析（PCA）系统地检查了 88 例 OS 病例的肿瘤微环境（TME）和免疫细胞浸润，并基于 TMEscore 高和 TMEscore 低表型构建了一个 TME 评分系统。我们的分析表明，TMEscore 高与 OS 患者的生存时间延长、免疫细胞浸润增加、免疫检查点增加以及对化疗的敏感性增加相关。TMEscore 低与免疫排斥强烈相关。我们使用来自 53 例 OS 患者的 GEO 数据集（GSE21257）进行了外部验证。我们的实验室数据也证明了我们的发现。这一发现增强了我们对 OS 中免疫景观的理解，并可能揭示新的靶向治疗策略。
