Department of Research & Development, Shenzhen Shiningbiotek Co., Ltd., Shenzhen, Guangdong, 518053, China.
Department of General Surgery, Liyuan Hospital, Tongji, Medical College, Huazhong University of Science & Technology, Wuhan, Hubei, 430077, China.
Biotechniques. 2022 Sep;73(3):151-158. doi: 10.2144/btn-2022-0029. Epub 2022 Sep 6.
Roche's AVENIO ctDNA analysis kits and bioinformatics analysis (the AVENIO system) are accessible to all NGS laboratories. We have developed an approach, namely the Sec-Seq system, and compared the accuracy, sensitivity, repeatability and economic cost between the AVENIO system and the Sec-Seq system. Both methods share the comparable accuracy and sensitivity in detecting the variant allele frequency of 0.0005, while the Sec-Seq system shows better accuracy in detecting the variant allele frequency of 0.001. Furthermore, the Sec-Seq system displays a much better detection sensitivity than the AVENIO system. The Sec-Seq system has satisfactory performance in detecting the rare genetic variants in ctDNA with lower economic cost compared with the AVENIO system.
罗氏的 AVENIO ctDNA 分析试剂盒和生物信息学分析（AVENIO 系统）对所有 NGS 实验室开放。我们开发了一种方法，即 Sec-Seq 系统，并比较了 AVENIO 系统和 Sec-Seq 系统在准确性、灵敏度、重复性和经济成本方面的差异。两种方法在检测 0.0005 的变异等位基因频率时具有相当的准确性和灵敏度，而 Sec-Seq 系统在检测 0.001 的变异等位基因频率时具有更好的准确性。此外，Sec-Seq 系统比 AVENIO 系统具有更好的检测灵敏度。Sec-Seq 系统在检测 ctDNA 中的稀有遗传变异方面具有令人满意的性能，与 AVENIO 系统相比，具有较低的经济成本。
