Kitabatake S, Dombou M, Tomioka I, Nakajima H
Biochem Biophys Res Commun. 1987 Jul 15;146(1):173-8. doi: 10.1016/0006-291x(87)90707-8.
A simple and practical procedure for the synthesis of P1,P4-di(adenosine 5'-) tetraphosphate from ATP by the catalysis of leucyl-tRNA synthetase from Bacillus stearothermophilus is described. Km for leucine was 6.7 microM and for ATP was 3.3 mM. The reaction yielded not only diadenosine tetraphosphate, but various byproducts such as P1,P3-(diadenosine 5'-) triphosphate, ADP and AMP. By coupling the reaction with an ATP regeneration system by acetate kinase and adenylate kinase with acetylphosphate as a phosphate donor, diadenosine tetraphosphate was prepared as a sole product at a high yield (96%).
描述了一种简单实用的方法，即通过嗜热脂肪芽孢杆菌的亮氨酰 - tRNA合成酶催化从ATP合成P1，P4 - 二（腺苷5'-）四磷酸。亮氨酸的Km为6.7微摩尔，ATP的Km为3.3毫摩尔。该反应不仅产生了二腺苷四磷酸，还产生了各种副产物，如P1，P3 - （二腺苷5'-）三磷酸、ADP和AMP。通过将该反应与由乙酸激酶和腺苷酸激酶组成的ATP再生系统偶联，并以乙酰磷酸作为磷酸供体，以高收率（96%）制备了唯一产物二腺苷四磷酸。
