Bello M, Thomas D, Legoy M D
Biochem Biophys Res Commun. 1987 Jul 15;146(1):361-7. doi: 10.1016/0006-291x(87)90733-9.
Unusual reactions of interesterification and synthesis catalyzed by Candida cylindracea lipase have been tested in reverse microemulsions. The microemulsions used are made of fatty acids or triglycerides, the enzyme dissolved in a very low water quantity, Brij 35 used as surfactant and an alcoholic cosurfactant. In such a system, fats and alcohols are both the substrates of the enzyme and the microemulsion components. Incidentally, non specific Candida cylindracea lipase does not catalyze interesterification of short chain triglycerides, revealing a specificity for the chain length. Interesterification reactions tested in the presence of a given water quantity but with varying water activities show that it is the water activity and not the water quantity which is a fundamental parameter of the system. The effect of the surfactant (Brij 35) on the interesterification reaction is studied. Heptyl-oleate synthesis catalyzed by non-specific lipase is obtained in microemulsions at a 98% yield. Synthesis of glycerol esters is also tested in monophasic medium and mono and diglycerides are obtained.
已在反相微乳液中测试了柱形假丝酵母脂肪酶催化的酯交换反应和合成反应的异常反应。所使用的微乳液由脂肪酸或甘油三酯制成，酶溶解在极少量的水中，使用Brij 35作为表面活性剂和醇类助表面活性剂。在这样的体系中，脂肪和醇既是酶的底物又是微乳液的成分。顺便提一下，非特异性柱形假丝酵母脂肪酶不催化短链甘油三酯的酯交换反应，这表明其对链长具有特异性。在给定水量但水活度不同的情况下测试的酯交换反应表明，水活度而非水量是该体系的基本参数。研究了表面活性剂（Brij 35）对酯交换反应的影响。在微乳液中，非特异性脂肪酶催化合成庚基油酸酯的产率为98%。还在单相介质中测试了甘油酯的合成，并得到了甘油单酯和甘油二酯。
