Iyyappan J, Jayamuthunagai J, Bharathiraja B, Saravanaraj A, Praveen Kumar R, Balraj S
Department of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha Nagar, Thandalam, Chennai 602107, India.
Centre for Biotechnology, Anna University, Chennai 600025, India.
Bioresour Technol. 2022 Nov;363:127893. doi: 10.1016/j.biortech.2022.127893. Epub 2022 Sep 5.
In this study, Caulerpa racemosa oil was used to produce biodiesel by recombinant Pichia pastoris displaying bound (rPp-BL) and secretory lipase (rPp-SL). Collected algae was pre-treated using ultrasonication, microwave and solvent extraction. Defatted C. racemosa was subjected to dilute acid treatment to obtain algal biomass hydrolysate. Both rPp-BL and rPp-SL were cultivated in algal biomass hydrolysate and glycerol. Surfactant treatment was performed on rPp-BL. Screening and optimization of variables were performed for biodiesel production using Plackett Burman design and central composite design, respectively. About 10.64 % (w/w) of algal oil was extracted from C. racemosa. Both rPp-BL and rPp-SL effectively utilized C. racemosa biomass hydrolysate and glycerol. rPp-SL combined with triton X (1.0 % w/v) treated rPp-BL for 3 min improved lipase activity. Methanol to oil ratio, combined whole cell biocatalyst and temperature were significant factors. Under optimum conditions, biodiesel yield reached about 93.64 % after 30 h using developed whole cell biocatalyst.
在本研究中，用重组毕赤酵母展示结合型脂肪酶（rPp-BL）和分泌型脂肪酶（rPp-SL）将总状蕨藻油用于生产生物柴油。收集的藻类采用超声处理、微波处理和溶剂萃取进行预处理。脱脂后的总状蕨藻进行稀酸处理以获得藻类生物质水解产物。rPp-BL和rPp-SL均在藻类生物质水解产物和甘油中培养。对rPp-BL进行表面活性剂处理。分别使用Plackett Burman设计和中心复合设计对生物柴油生产的变量进行筛选和优化。从总状蕨藻中提取了约10.64%（w/w）的藻油。rPp-BL和rPp-SL均能有效利用总状蕨藻生物质水解产物和甘油。rPp-SL与曲拉通X（1.0% w/v）联合处理rPp-BL 3分钟可提高脂肪酶活性。甲醇与油的比例、复合全细胞生物催化剂和温度是显著因素。在最佳条件下，使用所开发的全细胞生物催化剂，30小时后生物柴油产率达到约93.64%。
