巯基乙醇有助于将不含半胱氨酸/胱氨酸的蛋白质裂解为其亚基。
Mercaptoethanol assists in cleaving a cysteine/cystine-free protein into its subunits.
作者信息
Einhoff W, Kummer H, Rüdiger H
出版信息
Biol Chem Hoppe Seyler. 1987 Apr;368(4):405-7. doi: 10.1515/bchm3.1987.368.1.405.
PMID:3606824
Abstract
The alpha-mannosidase from Canavalia ensiformis is devoid of cysteine and cystine. Nevertheless, its cleavage into subunits is facilitated by the presence of mercaptoethanol in the denaturing buffer.
摘要
来自刀豆的α-甘露糖苷酶不含半胱氨酸和胱氨酸。然而，在变性缓冲液中存在巯基乙醇的情况下，它更容易裂解成亚基。
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