Department of Cardiology, Faculty of Medicine, Katip Çelebi University, İzmir, Turkey.
Department of Medical Biology, Faculty of Medicine, Ege University, İzmir, Turkey.
Turk Kardiyol Dern Ars. 2022 Sep;50(6):407-414. doi: 10.5543/tkda.2022.22408.
MicroRNAs have been explored as potential biomarkers for many pathological processes including coronary artery disease. In this study, we aimed to compare the circulating levels of selected atherosclerosis-associated miRNAs in patients with a history of early-onset coronary artery disease with that of age- and sex-matched healthy controls and older patients with late-onset coronary artery disease.
Study population consisted of 30 patients with early onset coronary artery disease, 31 age- and sex-matched healthy controls, and 30 patients with late-onset coronary artery disease. Plasma levels of 13 microRNAs (endothelial cell-related miR-126, -92a/b; vascular smooth muscle cell-related miR-145; inflammation-related miR-16, -21, -125b, -146a/b, -147b, -150, -155; lipometabolism-related miR-27b, -122, -370) were evaluated by using real-time polymerase chain reaction.
In patients with early onset coronary artery disease, plasma expressions of the lipometabolism-related miR-27b, miR-122; inflammation-related miR-125b, miR-146a/b, miR-147b, miR-150, miR-155; and VSMC-related miR-145 were significantly downregulated and endothelial cell-related miR-126 was significantly upregulated compared to age- and sexmatched healthy controls. Circulating microRNA profile of patients with early onset coronary artery disease was also different from that of older patients with late-onset coronary artery disease. Plasma levels of miR-21, miR-27b, miR-122, miR-125b, miR-146b, miR-147b, and miR-155 were lower and plasma levels of miR-16 and miR-92a were higher in patients with early onset coronary artery disease compared to older patients with late-onset coronary artery disease.
MicroRNAs are promising biomarkers for early onset coronary artery disease.
microRNAs 已被探索作为包括冠状动脉疾病在内的许多病理过程的潜在生物标志物。在这项研究中，我们旨在比较具有早发冠状动脉疾病病史的患者与年龄和性别匹配的健康对照者以及具有晚发冠状动脉疾病的老年患者的选定动脉粥样硬化相关 microRNAs 的循环水平。
研究人群包括 30 例早发冠状动脉疾病患者、31 例年龄和性别匹配的健康对照者以及 30 例晚发冠状动脉疾病患者。通过实时聚合酶链反应评估 13 种 microRNAs（内皮细胞相关的 miR-126、-92a/b；血管平滑肌细胞相关的 miR-145；炎症相关的 miR-16、-21、-125b、-146a/b、-147b、-150、-155；脂质代谢相关的 miR-27b、-122、-370）的血浆水平。
与年龄和性别匹配的健康对照者相比，早发冠状动脉疾病患者的脂质代谢相关的 miR-27b、miR-122；炎症相关的 miR-125b、miR-146a/b、miR-147b、miR-150、miR-155；和 VSMC 相关的 miR-145 的血浆表达明显下调，而内皮细胞相关的 miR-126 明显上调。早发冠状动脉疾病患者的循环 microRNA 谱也与老年晚发冠状动脉疾病患者不同。与老年晚发冠状动脉疾病患者相比，早发冠状动脉疾病患者的 miR-21、miR-27b、miR-122、miR-125b、miR-146b、miR-147b 和 miR-155 的血浆水平较低，而 miR-16 和 miR-92a 的血浆水平较高。
microRNAs 是早发冠状动脉疾病有希望的生物标志物。
