National Research Tomsk State University, 36 Lenin Ave, Tomsk 634050, Russia.
P.N. Lebedev Physical Institute, 53 Leninsky Prospect, Moscow 119333, Russia.
ACS Appl Mater Interfaces. 2022 Sep 21;14(37):41742-41750. doi: 10.1021/acsami.2c11027. Epub 2022 Sep 7.
We have explored the effect of low-temperature barrier discharge plasma treatment in oxygen, nitrogen, and argon on modification of the physicochemical properties of polylactic acid (PLA)-based scaffolds. The cellular-mediated immune response to the interaction of macrophages of three donors with the modified surface of PLA-based scaffolds was also investigated. Carbonization of the PLA surface accompanied by a carbon atomic concentration increase is shown to occur following plasma treatment. Argon plasma significantly affects the wettability characteristics of PLA; the hydrophilicity and lipophilicity are improved, and the surface energy is increased. The viability of cells in the presence of plasma-modified PLA scaffolds is lower than that for unmodified PLA but remains greater than that for the negative control. We find that PLA scaffolds do not cause increased expression of the proinflammatory (TNFα, IL-6, IL-1β) cytokines after 6 days of cell cultivation. At the same time, PLA scaffolds do not affect the increased production of anti-inflammatory cytokines (IL-10).
我们探索了低温阻挡放电等离子体处理在氧气、氮气和氩气中对聚乳酸（PLA）基支架物理化学性质的改性效果。还研究了三种供体的巨噬细胞与 PLA 基支架改性表面相互作用的细胞介导免疫反应。结果表明，等离子体处理后 PLA 表面发生碳化，碳原子浓度增加。氩等离子体显著影响 PLA 的润湿性特征；亲水性和疏水性得到改善，表面能增加。在存在等离子体改性 PLA 支架的情况下，细胞的存活率低于未改性 PLA，但仍高于阴性对照。我们发现 PLA 支架在细胞培养 6 天后不会引起促炎细胞因子（TNFα、IL-6、IL-1β）的表达增加。同时，PLA 支架不影响抗炎细胞因子（IL-10）的增加产生。
