Wang Luoluo, Pang Zhichang, Chen Qi, Song Zhentao, Lu Yi, Yang Meng, Huang Jia, Yu Xiao-Qiang, Wang Xiaoyun
Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou, China.
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
Insect Sci. 2023 Apr;30(2):486-500. doi: 10.1111/1744-7917.13116. Epub 2022 Sep 27.
Insecticides are anthropogenic environmental stressors and also a common stressor for mosquito vectors. However, the use of insecticides is often guided by short-term efficacy, and the sublethal effect on their target or nontarget species has long been ignored. Here, we analyzed how sublethal exposure of the promising vector-control bioinsecticide spinetoram to Aedes aegypti larvae alter adult performance and susceptibility to dengue virus (DENV) infection. We found that the surviving adult mosquitoes were significantly smaller and exhibited weaker blood-feeding capacity than control females, apart from the extended immature development period. In terms of reproductive potential, although the F generation produced a similar number of eggs and offspring during the first gonotrophic cycle, the survival rates of the F generations were significantly lower as compared to the control group, suggesting transgenerational sublethal effects on the F generation. Notably, surviving adult females had higher DENV-2 viral loads than the control group after spinetoram sublethal exposure. Mechanistically, transcriptomic analysis showed that inhibition of oxidative phosphorylation may function in stimulating DENV production in adult Ae. aegypti. In Aag2 cells, significant accumulation of apoptosis, mitochondrial reactive oxygen species production, and DENV-2 replication by spinetoram exposure consistently support our conclusion. Our study highlights the threat of sublethal spinetoram exposure on outbreaks of mosquito-borne viruses.
杀虫剂是人为环境应激源，也是蚊媒常见的应激源。然而，杀虫剂的使用往往以短期效果为导向，其对目标或非目标物种的亚致死效应长期以来一直被忽视。在此，我们分析了有前景的病媒控制生物杀虫剂多杀菌素对埃及伊蚊幼虫的亚致死暴露如何改变成虫性能以及对登革病毒（DENV）感染的易感性。我们发现，存活的成年蚊子明显更小，与对照雌蚊相比，除了未成熟发育期延长外，还表现出较弱的吸血能力。在生殖潜力方面，虽然F代在第一个生殖营养周期产生的卵和后代数量相似，但与对照组相比，F代的存活率显著较低，这表明对F代有跨代亚致死效应。值得注意的是，多杀菌素亚致死暴露后，存活的成年雌蚊的DENV - 2病毒载量高于对照组。从机制上讲，转录组分析表明氧化磷酸化的抑制可能在刺激成年埃及伊蚊的DENV产生中起作用。在Aag2细胞中，多杀菌素暴露导致的细胞凋亡显著积累、线粒体活性氧产生以及DENV - 2复制，始终支持我们的结论。我们的研究突出了多杀菌素亚致死暴露对蚊媒病毒爆发的威胁。
