University of Electronic Science and Technology of China, Chengdu 611731, China.
The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, The College of Laboratory Medicine, Chongqing Medical University, #1 Yixueyuan Road, Yuzhong District, Chongqing, 400016, China.
Analyst. 2022 Oct 10;147(20):4510-4516. doi: 10.1039/d2an01068b.
Serum alanine aminotransferase (ALT) is the most sensitive indicator of liver function; therefore, in clinical practice, its detection has diagnostic significance. However, hepatotoxicity monitoring is constrained in resource-limited areas due to the high cost and expensive equipment. This article describes a low-cost, compact blood enzyme analyzer (BEA) for point-of-care (PoC) liver function testing that uses reflection photometry to quantitate the colorimetric enzyme assay results. Moreover, the analyzer incorporates rapid and constant 37 °C temperature control to ensure that human serum enzymes remain active. The BEA was used to evaluate ALT in 50 whole blood samples using PoC photochemical test strips. The test results showed a linear correlation coefficient of 0.9749 compared with a clinical-specific biochemical analyzer and coefficients of variation (CV)% less than 5%. It has a low detection limit of 3.62 U L and a wide detection range of 4-480 U L. In addition, the BEA enables smartphone access to the Internet of Medical Things (IoMT) Bluetooth to facilitate active chronic disease management or remote diagnosis in PoC settings. Therefore, the BEA is a promising system for health management using the IoMT.
血清丙氨酸氨基转移酶（ALT）是肝功能最敏感的指标；因此，在临床实践中，其检测具有诊断意义。然而，由于成本高和设备昂贵，在资源有限的地区，肝毒性监测受到限制。本文描述了一种用于即时检测（POC）肝功能的低成本、紧凑型血液酶分析仪（BEA），该分析仪采用反射光度法对比色酶分析法的结果进行定量。此外，该分析仪采用快速且稳定的 37°C 温度控制，以确保人体血清酶保持活性。BEA 使用 POCT 光化学测试条评估了 50 份全血样本中的 ALT。与临床专用生化分析仪相比，测试结果显示出 0.9749 的线性相关系数，变异系数（CV）%小于 5%。它的检测下限为 3.62 U/L，检测范围为 4-480 U/L。此外，BEA 可通过智能手机访问物联网（IoT）蓝牙，以促进在 POCT 环境中进行主动慢性病管理或远程诊断。因此，BEA 是一个很有前途的 IoMT 健康管理系统。
