Department of Periodontology and Implantology, School of Dentistry, Federal University of Uberlândia, Avenida Pará s/n°, Campus Umuarama, Bloco 4L, Bairro Umuarama, Uberlândia, Minas Gerais, 38400-902, Brazil.
Department of Oral Diagnosis, School of Dentistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
J Digit Imaging. 2023 Feb;36(1):356-364. doi: 10.1007/s10278-022-00693-w. Epub 2022 Sep 7.
The objective of this study was to evaluate the influence of the extraction socket (distal or lingual root) and the type of region of interest (ROI) definition (manual or predefined) on the assessment of alveolar repair following tooth extraction using micro-computed tomography (micro-CT). The software package used for scanning, reconstruction, reorientation, and analysis of images (NRecon, DataViewer, CT-Analyzer) was acquired through Bruker < https://www.bruker.com > . The sample comprised the micro-CT volumes of seven Wistar rat mandibles, in which the right first molar was extracted. The reconstructed images were analyzed using the extraction sockets, i.e., the distal and intermediate lingual root and the method of ROI definition: manual (MA), central round (CR), and peripheral round (PR). The bone volume fraction (BV/TV) values obtained were analyzed by two-way ANOVA with Tukey's post hoc test (α = 5%). The distal extraction socket resulted in significantly lower BV/TV values than the intermediate lingual socket for MA (P = 0.001), CR (P < 0.001), and PR (P < 0.001). Regarding the ROI, when evaluating the distal extraction socket, the BV/TV was significantly higher (P < 0.001) for MA than for CR and PR, with a lower BV/TV for CR. However, no significant difference was observed for MA (P = 0.855), CR (P = 0.769), or PR (P = 0.453) in the intermediate lingual extraction socket. The bone neoformation outcome (BV/TV) for alveolar bone repair after tooth extraction is significantly influenced by the ROI and the extraction socket. Using the predefined method with a standardized ROI in the central region of the distal extraction socket resulted in the assessment of bone volume, demonstrating the most critical region of the bone neoformation process.
本研究的目的是评估在使用微计算机断层扫描（micro-CT）评估拔牙后牙槽骨修复时，提取窝（远中或舌侧根）和感兴趣区域（ROI）定义类型（手动或预定义）的影响。用于扫描、重建、重新定向和分析图像的软件包（NRecon、DataViewer、CT-Analyzer）是通过 Bruker 公司获得的https://www.bruker.com。样本包括 7 只 Wistar 大鼠下颌骨的 micro-CT 体积，其中右侧第一磨牙被拔出。使用提取窝（即远中舌侧根和中间舌侧根）和 ROI 定义方法（手动（MA）、中央圆形（CR）和外周圆形（PR））对重建图像进行分析。使用双向方差分析和 Tukey 事后检验（α=5%）对获得的骨体积分数（BV/TV）值进行分析。对于 MA（P=0.001）、CR（P<0.001）和 PR（P<0.001），远中提取窝的 BV/TV 值明显低于中间舌侧窝。对于 ROI，在评估远中提取窝时，MA 的 BV/TV 值明显高于 CR（P<0.001）和 PR（P<0.001），而 CR 的 BV/TV 值较低。然而，在中间舌侧提取窝中，MA（P=0.855）、CR（P=0.769）或 PR（P=0.453）之间无显著差异。拔牙后牙槽骨修复的新骨形成结果（BV/TV）受 ROI 和提取窝的影响显著。在远中提取窝的中央区域使用预定义方法和标准化 ROI 评估骨体积，可显示新骨形成过程的最关键区域。
